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| Research on the lipid-lowering mechanism through the PCSK9/LDLR pathway by Danzhi Qing’e Formula |
| Hits 66 Download times 32 Received:April 20, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.07.13 |
| Key Words
Danzhi Qing’e Formula;dislipidemia;proprotein convertase subtilisinkexin type 9;mechanism |
| Author Name | Affiliation | E-mail | | SONG Junli | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SHI Huihui | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHEN Guangxia | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | HUANG Yuxing | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | MAO Haoping | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | maohaoping@tjutcm.edu.cn | | MA Shangwei | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | ivyer1314@126.com |
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| Abstract
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| [Objective] To explore the lipid-lowering mechanism of Danzhi Qing’e Formula（DZQEF）based on network pharmacology， molecular docking and experimental studies. [Methods] The uptake of Dil-LDL by HepG2 cells treated with Danzhi Qing’e Formula was detected. The mechanism of Danzhi Qing’e Formula in treating hyperlipidemia was predicted by network pharmacology and preliminarily verified by molecular docking. A hyperlipidemia rat model was established to detect the levels of serum total cholesterol （TC），triglyceride（TG），low-density lipoprotein cholesterol（LDL-C），high-density lipoprotein cholesterol（HDL-C），aspartate aminotransferase（AST）and alanine aminotransferase（ALT）. The protein expressions of proprotein convertase subtilisin/kexin type 9 （PCSK9）and low-density lipoprotein receptor （LDLR）in the liver of hyperlipidemia rats were determined by Western blot. [Results] Cell experiments showed that 100 μg/mL Danzhi Qing’e Formula significantly enhanced the Dil-LDL uptake ability of HepG2 cells（P< 0.01）. A total of 2 543，4 376，1 396 and 3 217 targets were screened out for the effective active components of Eucommia ulmoides， Salvia miltiorrhiza，Anemarrhena asphodeloides and Psoralea corylifolia，respectively. There were 2 061 targets related to hyperlipidemia. There were 324 common targets between Danzhi Qing’e Formula and hyperlipidemia. Gene Ontology（GO）analysis was involved in the cholesterol metabolism process，and Kyoto Encyclopedia of Genes and Genomes（KEGG）was mainly related to lipid and atherosclerosis pathways，both of which included the LDLR gene. Molecular docking indicated that PCSK9 had good docking activity with the core active targets. In vivo experiments showed that 10 g/kg Danzhi Qing’e Formula could effectively reduce LDL-C， TC，TG，AST and ALT levels of hyperlipidemia rats，as well as the protein expressions of PCSK9，and significantly up-regulate the protein expressions of LDLR（P<0.01）. [Conclusion] Danzhi Qing’e Formula may exert lipid-lowering effects by regulating the PCSK9/LDLR signaling pathway. |
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