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| Effects of acupuncture at "Baihui"(GV20) through "Qubin"(GB7) on the expression levels of mitochondrial fission and fusion-related proteins in rats with intracerebral hemorrhage |
| Hits 295 Download times 127 Received:May 06, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.10.14 |
| Key Words
intracerebral hemorrhage;acupuncture;mitochondrial fission;mitochondrial fusion;Baihui;Qubin |
| Author Name | Affiliation | E-mail | | OU Yuyang | Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China | | | LI Yunong | Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China | | | LUO Yaoxing | Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China | | | ZHANG Yixin | Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China | | | LI Dan | Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China | 3509437@qq.com |
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| Abstract
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| Objective To explore the effects and mechanisms of acupuncture at "Baihui"(GV20) through "Qubin"(GB7) on intracerebral hemorrhage(ICH) by examining mitochondrial fission proteins(Drp1, its activated forms, and Fis1) and mitochondrial fusion proteins(Mfn1, Mfn2, and OPA1).Methods Thirty SPF-grade male SD rats were randomly divided into a sham group, an ICH group, and an ICH + acupuncture group(n=10 per group). The ICH model was established in the latter two groups using the autologous blood injection method into the striatum, and successful modeling was assessed with the Bederson neurological score. One-hour post-modeling, acupuncture treatment was initiated for the ICH+acupuncture group, performed once daily for 30 minutes over 3 consecutive days. Neurological function was re-evaluated after the last acupuncture session, followed by tissue sampling. Transmission electron microscopy was used to observe the morphology, size, and quantity of mitochondria in the peri-hematoma region. Real-time PCR was employed to measure mitochondrial DNA(mtDNA) expression levels. Western blot analysis was conducted to detect the expression of mitochondrial fission proteins p-Drp1(Ser637), p-Drp1(Ser616), mito-Drp1, cyto-Drp1, Fis1, mitochondrial fusion proteins Mfn1, Mfn2, and OPA1, as well as p-p38α in the MAPK/Drp1 pathway.Results Compared with the ICH group, the ICH+acupuncture group exhibited significantly improved Bederson neurological scores on the final day of acupuncture(P < 0.000 1). Transmission electron microscopy revealed that mitochondrial morphology was markedly abnormal in the ICH group but improved in the ICH+acupuncture group. The mtDNA expression levels were significantly decreased in the ICH group compared to the sham group(P < 0.000 1) and significantly increased in the ICH + acupuncture group compared to the ICH group(P < 0.000 1). Expression of p-Drp1(Ser637) and cyto-Drp1 was reduced in the ICH group compared to the sham group(P < 0.000 1) but increased in the ICH+acupuncture group compared to the ICH group(P < 0.000 1). Conversely, expression of p-Drp1(Ser616), mito-Drp1, and Fis1 was elevated in the ICH group compared to the sham group(P < 0.000 1) but decreased in the ICH+acupuncture group compared to the ICH group(P < 0.000 1). Expression levels of Mfn1 and Mfn2 were significantly reduced in the ICH group compared to the sham group(P < 0.000 1) and significantly increased in the ICH+acupuncture group compared to the ICH group(P < 0.000 1). Expression levels of OPA1 S/L were elevated in the ICH group compared to the sham group(P < 0.000 1), but decreased in the ICH+acupuncture group compared to the ICH group(P < 0.000 1).Conclusion Acupuncture at Baihui through Qubin alleviates neurological damage following ICH by inhibiting abnormal mitochondrial fission, promoting mitochondrial fusion, and maintaining mitochondrial dynamics and genetic integrity. |
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