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| Mechanism of Shenzhu Xinkang Decoction alleviating adriamycin-induced myocardial cell injury by regulating Wnt/β-catenin signal pathway |
| Hits 308 Download times 115 Received:July 13, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.11.14 |
| Key Words
Shenzhu Xinkang Decoction;Wnt/β-catenin signal pathway;adriamycin;apoptosis;oxidative stress;cardiac muscle cells |
| Author Name | Affiliation | E-mail | | ZHU Xiaojing | Department of Cardiology, Hunan Integrated Traditional Chinese and Western Medicine Hospital, Changsha 410006, China | | | JIANG Yang | Department of Gastroenterology, Hunan Integrated Traditional Chinese and Western Medicine Hospital, Changsha 410006, China | | | YU Zhengke | Department of Cardiology, Hunan Integrated Traditional Chinese and Western Medicine Hospital, Changsha 410006, China | | | YAN Xu | Department of Cardiology, Hunan Integrated Traditional Chinese and Western Medicine Hospital, Changsha 410006, China | cathy_july@163.com |
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| Abstract
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| [Objective] To investigate whether Shenzhu Xinkang Decoction（SZXK） can alleviate adriamycin（Dox） -induced myocardial cell injury by regulating Wnt/β-catenin signal pathway. [Methods] Rat H9c2 cardiomyocytes were cultured in vitro and divided into the following groups：control group，Dox group（H9c2 cells were stimulated with 1 μmol/L Dox for 24 h），SZXK-L group（low dose，20 μmol/L），SZXK-M group（medium dose，40 μmol/L），SZXK-H group（high dose，80 μmol/L），SZXK-H+M group（MSAB，Wnt/β-catenin pathway inhibitor）. The cytotoxicity of SZXK on H9c2 cells was detected by methyl thiazol tetrazolium（MTT） assay，cell viability was detected by cell counting kit-8（CCK-8），and cell apoptosis was detected by flow cytometry. Western blot was used to detect the expression levels of B-cell lymphoma-2（Bcl-2），Bcl-2 associated X protein（Bax） and Wnt/β-catenin signal pathway proteins. The levels of tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），interleukin-6（IL-6），reactive oxygen species（ROS），superoxide dismutase（SOD） and malondialdehyde（MDA） were detected by kits. [Results] Compared with the control group，the cell viability，Bcl-2 and SOD levels in the model group were significantly decreased，while the apoptosis rate，Bax level，ROS，MDA，IL-6，TNF-α and IL-1β levels were increased（P<0.05），indicating that the model of myocardial cell injury was successfully established. Compared with the model group，SZXK dose-dependently increased cardiomyocyte viability，inhibited cell apoptosis，and alleviated oxidative stress injury and inflammatory response. In addition，compared with the Control group，the levels of Wnt3a and β-catenin nuclear protein in the Dox group were significantly decreased，and the level of β-catenin protein was increased（P<0.05）. After SZXK treatment，the Wnt3a and β-catenin nuclear protein levels were significantly increased，and the β-catenin protein level was decreased in a dose-dependent manner（P<0.05）. Inhibition of Wnt/β-catenin pathway partially reversed the protective effect of SZXK on DOX-induced myocardial cell injury. [Conclusion] SZXK may exert a protective effect on DOX-induced cardiomyocyte injury by activating the Wnt/β-catenin signal pathway. |
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