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| Study on potential action mechanism of Yupingfeng teabags in treatment of Hashintoto’s thyroditis based on UPLC-Q-TOF/MS |
| Hits 375 Download times 245 Received:March 14, 2005 |
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| DOI
10.11656/j.issn.1672-1519.2025.07.12 |
| Key Words
component analysis;network pharmacology;mechanism of action;Hashintoto’s thyroditis;Yupingfeng teabags |
| Author Name | Affiliation | E-mail | | DU Qunqun | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China
Guangzhou Parmaceutical Holddings Limited, Guangzhou 510515, China
The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510515, China | | | WANG Xiaolan | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China | | | ZHENG Xiaoling | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China | | | ZHANG Yongtao | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China | | | HE Fenglei | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China | 13711627207@163.com | | QIN Renan | Research and Development Centre, Guangzhou Baiyunshan Chenliji Parmaceutical Co., Ltd., Guangzhou 510515, China | zhangying30412@163.com | | ZHANG Ying | The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510515, China | |
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| Abstract
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| [Objective] To explore the regulatory mechanism of Yupingfeng teabags in treatment of Hashintoto’s thyroditis through UPLC-Q-TOF/MS and network pharmacology. [Methods] UPLC-Q-TOF/MS was used to qualitatively analyze the chemical components of Yupingfeng teabags. Network pharmacology was used to predict the potential targets of Yupingfeng teabags in treatment of Hashintoto’s thyroditis. And animal experiments were used to verify the therapeutic effect of Yupingfeng teabags on Hashintoto’s thyroditis. [Results] Totally 75 compounds were identified. The results of Network pharmacology showed that the effect of Yupingfeng teabags on treatment of Hashintoto’s thyroditis might be related to calcium signaling pathway，VEGF signaling pathway，Rap1 signaling pathway，IL-17 signaling pathway，MAPK signaling pathway and other signaling pathways. Animal experiments showed that Yupingfeng teabags significantly reduced the levels of TGAb，TPOAb and TSH in Hashimoto’s thyroiditis model mice，increased the levels of FT3 and FT4，and maintained the stability of thyroid hormones and antibodies. Moreover，Yupingfeng teabags protected the integrity of follicular cells in thyroid tissue，and reduced lymphocyte infiltration，and the expression of IL-17，VEGF and TNF-α protein in thyroid tissue. [Conclusion] The pharmacodynamic basis of Yupingfeng teabags is determined by UPLC-Q-TOF/MS. Its potential mechanism for Hashintoto’s thyroditis is made clear by being combination with network pharmacology and animal experiments，providing reference for the development and utilization of Yupingfeng teabags. |
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