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| Study on the dynamic changes of synthesis and accumulation of 8 main active ingredients in glycyrrhiza germplasm from 3 different germplasm sources |
| Hits 396 Download times 181 Received:April 18, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.09.12 |
| Key Words
Glycyrrhiza uralensis;harvest period;high-performance liquid chromatography;total flavonoids;total polysaccharides |
| Author Name | Affiliation | E-mail | | WANG Xuna | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Qi | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Naijiao | China Medico Corporation, Tianjin 300301, China | | | CHEN Yanguo | China Medico Corporation, Tianjin 300301, China | | | WANG Xueyuan | China Medico Corporation, Tianjin 300301, China | | | YUAN Hongyu | China Medico Corporation, Tianjin 300301, China | | | LI Guohui | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Tianxiang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | litianxiang612@sina.com |
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| Abstract
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| [Objective] The study focuses on the introduction of three wild varieties of Glycyrrhiza uralensis，Hangjin Banner，Chifeng，and Huining，from the licorice authentic production areas in China，into Tianjin. Samples were collected every 15 days during the one-year growth period to determine the content of eight main functional components. The synthesis and accumulation patterns and characteristics of active components in medicinal materials of different qualities and harvesting periods of licorice from the three production areas were systematically investigated，and excellent sources were screened. [Methods] Collect samples every half month，and use HPLC，NaNO2-Al（NO3）3-NaOH colorimetric method，and anthrone H2SO4 method to determine the content of 8 main active ingredients in samples at different harvesting periods. Investigate the dynamic changes and patterns of the synthesis and accumulation of active ingredients. [Results] The changes in the synthesis and accumulation of liquiritigenin，glycyrrhetinic acid，isoliquiritigenin，total flavonoids， and total polysaccharides in three germplasm licorice samples introduced to Tianjin at different harvesting periods were basically consistent. However，the content of liquiritin，glycyrrhetinic acid，and isoliquiritin Huining licorice was higher than that in Hangjinqi and Chifeng licorice at different harvesting times. [Conclusion] Three wild germplasm of Glycyrrhiza uralensis have good growth adaptability after being introduced to Tianjin. During the one-year growth cycle，there is no significant difference in the dynamic synthesis trend of the main active functional substances and functional components in licorice at different harvest periods. The Huining germplasm of licorice has a high level of cumulative synthesis of functional components and strong excellent germplasm characteristics. Therefore，the large-scale introduction of Huining licorice in Tianjin’s saline alkali soil environment has obvious potential. |
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