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| Protective effects of sodium houttuyfonate on acute lung injury in mice and its impact on gut microbiota |
| Hits 321 Download times 146 Received:April 26, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.09.13 |
| Key Words
acute lung injury;sodium houttuyfonate;gut microbiota;inflammation |
| Author Name | Affiliation | E-mail | | ZHENG Chunlong | Department of Thoracic Surgery, Second Affiliated Hospital of Air Force Military Medical University, Xi'an 710038, China | | | DUAN Wanshi | Department of Thoracic Surgery, Second Affiliated Hospital of Air Force Military Medical University, Xi'an 710038, China | drstoneduan@163.com |
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| Abstract
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| [Objective] To investigate the effects of sodium houttuyfonate（SH） on gut microbiota in a mouse model of lipopolysaccharide（LPS）-induced acute lung injury（ALI） and explore its potential mechanisms of action. [Methods] Eighteen male SPF-grade BALB/c mice（6~8 weeks old，weighing 18~22 g） were randomly divided into three groups：control，model（LPS），and SH（LPS+SH），with six mice in each group. The control and LPS groups received oral gavage of normal saline，while the SH group received SH（100 mg/kg） once daily. Except for the control group，the model and SH groups were intratracheally injected with 5 mg/kg LPS after 7 days of continuous treatment to establish the ALI model. Mice were weighed before and after modeling. At the end of the experiment，lung wet-to-dry（W/D） ratios were measured，histopathological changes were observed using hematoxylin and eosin（HE） staining，inflammatory cytokine levels were assessed by enzyme-linked immunosorbent assay（ELISA），protein expression levels in the JNK/p38 signaling pathway were determined by Western blot，and gut microbiota composition was analyzed using 16S rRNA sequencing. [Results] SH reduced the W/D ratio，improved histopathological damage in lung tissue，and inhibited myeloperoxidase（MPO） activity，tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），and interleukin-6（IL-6） levels in lung tissue. It also suppressed the expression of p-JNK and p-p38 proteins. SH significantly enhanced the α and β diversity of gut microbiota in ALI mice. The relative abundance of Firmicutes increased，while that of Proteobacteria decreased. At the genus level，the model group exhibited higher relative abundances of unidentified_Enterobacteriaceae，Bacteroides and Klebsiella，and lower abundances of Ligilactobacillus，Lactobacillus and Akkermansia，whereas the opposite trend was observed in the SH group. Inflammatory markers and pulmonary edema positively correlated with Proteobacteria，Klebsiellaand g_unidentified_Enterobacteriaceae，and negatively correlated with Akkermansia，Lactobacillus and Ligilactobacillus. [Conclusion] SH exerts protective effects against LPS-induced ALI，potentially through inhibiting inflammatory responses mediated by the JNK/p38 signaling pathway and restoring gut microbiota balance. |
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