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| Mechanism study on Jianpi Yanggan Pill in regulating enterochromaffin cells and TPH1-5-HT signaling pathway in treating IBS-D rats |
| Hits 379 Download times 192 Received:April 28, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.09.15 |
| Key Words
diarrheal irritable bowel syndrome;visceral hypersensitivity;Jianpi Yanggan Pill;enteroendocrine cell;5-hydroxytryptamine;Chromogranin A |
| Author Name | Affiliation | E-mail | | ZHU Ting | Yunnan University of Chinese Medicine, Kunming 650021, China
Department of Gastroenterology, Huize County Hospital of Traditional Chinese Medicine, Huize 654200, China | | | CHEN Xin | Yunnan University of Chinese Medicine, Kunming 650021, China | | | ZHAO Xin | Yunnan University of Chinese Medicine, Kunming 650021, China | | | HU Xueting | Yunnan University of Chinese Medicine, Kunming 650021, China | | | XIE Xiang | Yunnan University of Chinese Medicine, Kunming 650021, China | | | LUAN Yunpeng | Yunnan University of Chinese Medicine, Kunming 650021, China | | | LI Xiang | Yunnan University of Chinese Medicine, Kunming 650021, China | | | WANG Huaning | The First Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming 650500, China | | | MA Ke | The First Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming 650500, China | 406909561@qq.com |
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| Abstract
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| [Objective] To explore Jianpi Yanggan Pills alleviate visceral hypersensitivity（VH） in IBS-D by regulating the secretion of 5-hydroxytryptamine（5-HT） in enteroendocrine cells（EC），thereby revealing the visceral hypersensitivity mechanism of its treatment for IBS-DBy studying the effect of Jianpi Yanggan Pills on the expression of the enteroendocrine cells/5-hydroxytryptamine（EC/5-HT） signaling pathway in diarrhea-type irritable bowel syndrome（IBS-D） model rats. [Methods] An IBS-D rat model was established by using the mother-infant separation combined with restraint stress method. The efficacy of the IBS-D model and Jianpi Yanggan Pills was evaluated through fecal scoring，fecal water content determination，diarrhea index detection，and visceral sensitivity measurement. ELISA experiments，qPCR experiments，immunohistochemical staining，immunofluorescence staining，and electron microscopy were used to detect the synthesis and secretion of 5-HT in EC. [Results] Compared with the model group，the high，medium and low-dose Jianpi Yanggan Pills groups decreased the Bristol stool form score，fecal water content，visceral sensitivity，colonic and serum 5-HT levels，and the relative mRNA expression of TPH1 in IBS-D rats（P<0.05，P<0.01，P<0.001），alleviated colonic tissue pathological damage，reduced Chromogranin A positive expression，and through the treatment of Jianpi Yanggan Pills，ECs presented an oval shape and the number of secretory granules decreased. [Conclusion] Jianpi Yanggan Pills may reduce visceral hypersensitivity by decreasing TPH1-mediated EC reuptake of tryptophan to form 5-hydroxytryptophan（5-HTP），thereby reducing 5-HT production. |
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