|
| Effect of andrographolide on the activity and chemotherapy resistance of rectal cancer cells by regulating the SHH/GLI1 signaling pathway |
| Hits 233 Download times 39 Received:January 21, 2026 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2026.03.11 |
| Key Words
rectal cancer;andrographolide;SHH/GLI1 signaling pathway;chemotherapy resistance |
| Author Name | Affiliation | E-mail | | LIU Kun | Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao 266042, China | | | LIU Lihua | Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao 266042, China | | | HOU Weijing | Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao 266042, China | | | LU Lin | Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao 266042, China | | | ZHANG Meihua | Qingdao Central Hospital, University of Health and Rehabilitation Sciences, Qingdao 266042, China | tdnqxhox@163.com |
|
| Abstract
|
| [Objective] To investigate the effect of andrographolide on the activity and chemotherapy resistance of rectal cancer cells by regulating the sonic hedgehog（SHH）/GLI family zinc finger protein 1（GLI1） signaling pathway. [Methods] Human colorectal cancer drug-resistant cell line SW20/5-FU was treated with 3.75 to 120 μmol/L of andrographolide，and cell proliferation activity was detected. The experimental concentration of andrographolide was screened. SW20/5-FU cells were randomly divided into Control group，low，medium，and high concentration andrographolide groups（AND-L group，AND-M group，AND-H group），and high concentration andrographolide + SHH signaling pathway inhibitor group（Cyclopamine group）. The proliferation，migration，and invasion abilities of SW20/5-FU cells were detected. Epithelial mesenchymal transition（EMT） and pathway protein expression were detected. [Results] By detecting cell proliferation activity，the concentrations of andrographolide at 15.0，30.0，and 60.0 μmol/L were selected as the treatment concentrations. Compared with the Control group，the number of cell clones formed，scratch healing rate，and number of invasions decreased in AND-L，AND-M，and AND-H groups，while the expression levels of G1/S-specific cyclin D1（Cyclin D1），matrix metalloproteinase-2（MMP-2），matrix metalloproteinase-9（MMP-9），neural cadherin（N-Cadherin），and Vimentin decreased，the expression levels of epithelial cadherin（E-Cadherin），SHH，and GLI1 proteins increased（P<0.05）. Compared with the AND-H group，the number of cell clones formed，scratch healing rate，and number of invasions increased in Cyclopamine group，while the expression levels of CyclinD1，MMP-2，MMP-9，N-Cadherin，and Vimentin increased，the expression levels of E-Cadherin，SHH，and GLI1 proteins decreased（P<0.05）. [Conclusion] Andrographolide activates the SHH/GLI1 signaling pathway to inhibit the proliferation，migration，invasion，and EMT ability of rectal cancer cells，thereby improving chemotherapy resistance. |
|
|
|
|
|
|
|
