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| Mechanistic study on stress-modulated regulation of knee osteoarthritis via shaking manipulation-induced synovial cell inflammatory signaling pathways |
| Hits 158 Download times 31 Received:October 13, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2026.03.12 |
| Key Words
shaking manipulation;KOA;synovitis;FLSs;signaling pathway;stress |
| Author Name | Affiliation | E-mail | | WANG Yizhou | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | LONG Hailun | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | XING Anze | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | | | ZHAO Qiang | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | ywy0707@sina.com |
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| Abstract
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| [Objective] This study aimed to investigate the mechanism by which shaking manipulation alleviates synovial inflammation in knee osteoarthritis（KOA） by regulating the αVβ3/FAK/ERK/C-Jun signaling pathway. [Methods] A total of 100 male New Zealand white rabbits were randomly divided into five groups（Normal，Sham，Model，Shaking，and Celecoxib；n=20 per group）. The KOA model was induced by intra-articular injection of papain. The Shaking group received shaking manipulation treatment at a frequency of 0.5 Hz for 5 min once daily，and the Celecoxib group received intragastric administration of celecoxib solution，7 days per cycle for 3 cycles. Transmission electron microscopy was used to observe ultrastructural changes of synovial cells. Protein expression of αVβ3，FAK，ERK，and C-Jun was detected by Western blot，and mRNA expression was analyzed by RT-qPCR. Primary fibroblast-like synoviocytes（FLSs） were isolated using the tissue explant method. Levels of MMP-1 and MMP-13 in the culture supernatant were determined by radioimmunoassay，and F-actin cytoskeletal remodeling was observed by fluorescence staining. [Results] Shaking manipulation significantly improved synovial cell ultrastructure，downregulated the protein expression of αVβ3，FAK，ERK，and C-Jun（P<0.01），and reduced their mRNA levels（P<0.05）. The secretion of MMP-1 and MMP-13 was significantly decreased（P<0.01）. F-actin cytoskeletal remodeling was negatively correlated with inflammatory mediator secretion（P<0.05）. [Conclusion] Shaking manipulation improves KOA synovitis by regulating the αVβ3/FAK/ERK/C-Jun signaling pathway，inducing cytoskeletal remodeling in synovial cells，and suppressing MMP secretion. This study provides molecular-level evidence for the efficacy of manipulation therapy in KOA and highlights the role of mechanical stress in synovial inflammation regulation. |
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