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| Effects of Erteng Sanjie Capsule on intestinal microbiota in colorectal cancer cachexia mice based on 16S rDNA sequencing |
| Hits 144 Download times 20 Received:October 22, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2026.03.14 |
| Key Words
gut microbiota;cancer cachexia;Erteng Sanjie Capsule;CT26;Balb/C mice |
| Author Name | Affiliation | E-mail | | PEI Zhengying | Shanxi Institute of Traditional Chinese Medicine, Taiyuan 030012, China | | | FAN Duo | Shanxi University of Chinese Medicine, Jinzhong 030619, China | | | WANG Zhen | Shanxi University of Chinese Medicine, Jinzhong 030619, China | | | DONG Liqin | Oncology Department, Shanxi Traditional Chinese Medicine Hospital, Taiyuan 030012, China | | | HAO Shulan | Oncology Department, Shanxi Traditional Chinese Medicine Hospital, Taiyuan 030012, China | dxl328321@163.com |
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| Abstract
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| [Objective] To establish a mouse model of cancer cachexia by subcutaneous inoculation of CT26 colon cancer cells，and to investigate the effects of Erteng Sanjie Capsules on tumor-free body weight，gastrocnemius muscle mass，histopathological morphology of the gastrocnemius muscle，and changes in the gut microbiota structure. [Methods] Forty SPF-grade male Balb/C mice were randomly divided into normal group，model group，low-dose Erteng Sanjie Capsule group [0.75 g/（kg·d）]，medium-dose group [1.5 g/（kg·d）]，and high-dose group [3 g/（kg·d）]. Except for normal group，all other groups were subcutaneously inoculated with CT26 colon cancer cells. After 14 days of gavage，the body weight of the mice was measured，and fecal samples were collected before euthanasia. Tumor，liver，spleen，and gastrocnemius muscle tissues were collected and weighed. Part of the gastrocnemius muscle was subjected to HE staining to observe pathological changes，and fecal samples were analyzed by 16S rDNA high-throughput sequencing. [Results] Erteng Sanjie Capsules increased the tumor-free body weight and gastrocnemius muscle mass in mice with cancer cachexia，ameliorated muscle atrophy，enriched the diversity of gut microbiota，and modulated the microbial composition closer to that of the normal group. The relative abundances of un_f_Clostridiales_vadinBB60_group，Parabacteroides，Ruminococcaceae_UCG-014，Lactobacillus，and un_o_Mollicutes_RF39 were significantly increased.[Conclusion] Erteng Sanjie Capsules can ameliorate muscle loss，weight reduction，and gut microbiota dysbiosis induced by cancer cachexia. |
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