| 摘要: |
| 目的:采用薄层扫描法测定何首乌中大黄素的含量。方法:用索氏提取法,以氯仿为提取溶剂,展开剂为正己烷-醋酸乙酯-氯仿-甲酸(10:3:3:025),检测波长为440nm。结果:大黄素在0.1～5.0μg范围内线性关系良好(r=0.9995),平均回收率为105.73%,RSD为2.43%,斑点颜色在4h内基本稳定。结论:本法为控制何首乌的内在质量提供了依据。 |
| 关键词: 索氏提取 何首乌 大黄素 薄层锯齿扫描 |
| DOI：10.11656/j.issn.1672-1519.2002.03.29 |
| 分类号: |
| 基金项目:天津市科委重大科技攻关项目 |
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| Content Analysis of Emodin in Radix Polygoni Multiflori |
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Qi Aidi,Pan Shuowen,Zhu Chen
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Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| Objective: To analyze the content of emodin in Radix Polygoni Multiflori by thin-layer scanning. Methods: Emodin was distilled with Soxhlet extraction process, using CHCl3 as liquor, Skellysolve B-ethyl acetate-CHCl3-HCOOH (10: 3: 3: 0.25) as developer and detecting wavelength 440nm. Results: The linearity of emodin was fine in 0.1～5.0μg(r=0.9995), average recovery rate 105.73%, RSD 2.43%, color dot stable for 4 hours. Conclusion: The method had laid the foundation for the control of the inherent quality in Radix Polygoni Multiflori. |
| Key words: Soxhlet extraction Radix Polygoni Multiflori Emodin Thin-layer scanning |
