| 摘要: |
| [目的]探讨丹酚酸B对心脏微血管内皮细胞(CMEC)抗缺氧损伤的保护机制。[方法]基于缺氧预适应的保护机制,通过缺氧/复氧的CMEC损伤模型,采用分子生物学方法,观察蛋白激酶CmRNA表达情况。[结果]丹酚酸B预适应可促进缺氧/复氧损伤的CMEC的蛋白激酶CmRNA表达增强,且明显优于缺氧预适应(HPC)。[结论]丹酚酸B预适应与HPC具有相类似的细胞保护效应,可增强细胞对随后较长时间缺氧/复氧损伤的耐受性,其机制可能是通过增强蛋白激酶CmRNA表达实现的。 |
| 关键词: 丹酚酸B预适应 心脏微血管内皮细胞 蛋白激酶C |
| DOI：10.11656/j.issn.1672-1519.2004.04.29 |
| 分类号: |
| 基金项目:天津市自然科学基金资助项目 (013612711) |
|
| Effect of salvianolic acid B advanced adaptation on expression of protein kinase C-mRNA of the hypoxia and ambohypoxia impairing cardiac capillaries endothelial cell |
|
GUO Li-ping,DU Rong,ZHANG Meng
|
|
The First Afflicated Hospital to Tianjin University of TCM, Tianjin300193, China
|
| Abstract: |
| [Objective] To approach the mechanism of salvianolic acid B in protecting cardiac capillaries endothelial cell(CMEC)against hypoxia.[Methods] Based on the protective mechanism of the advanced adaptation,the expression of protein kinase C-mRNA was observed with molecular biology method,and the protective mechanism of salvianolic acid B on cells was approached.[Result] The expression of C-mRNA was upregulated in salvianolic acid B treated group and better than that in surpassed hypoxia advanced adaptation group(HPC).[Conclusion] Salvianolic acid B advanced adaptation has a similar cellular protective effect as HPC.It can enhance the tolerance longer hypoxia and ambohypoxia.The mechanism may be produced by related to the expressions of the protein kinase C-mRNA. |
| Key words: salvianolic acid B advanced adaptation cardiac capillaries endothelial cell(CMEC) protein kinase C-mRNA |
