| 摘要: |
| [目的]改进大黄药材中总蒽醌的含量测定方法。[方法]1,8-二羟基蒽醌与碱显色后,2h内隔时扫描光谱图;对照品和样品同样处理后绘制标准曲线。[结果]确定了最大吸收波长,消除了测定过程中样品湿热降解带来的误差。[结论]通过方法学考察,证明此方法精密准确、回收率高,可以作为大黄中总蒽醌的含量测定方法。 |
| 关键词: 大黄 总蒽醌 分光光度法 最大吸收波长 降解 |
| DOI：10.11656/j.issn.1672-1519.2004.04.33 |
| 分类号: |
| 基金项目:河北省医药开发重大项目 (No:02276408D-2) |
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| Inprovement of determing method total Rehum Anthrapuinone in Rehum Herb by visible-spectophotometric |
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LIU Cui-zhe, WANG Ru-xing, LUI Xi-gang
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Institute of Chinese Materia Medica, Chengde Medical College, Chengde06700, China
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| Abstract: |
| [Objective] To improve the determining method of total Rehum anthrapuinone in Rehum herb by visible-spectrophotometric.[Methods] Scanning the spectrum interval in two hours after the coloration of1.8-dihydro- anthrapuine and alkine.The standard curbe was drawn after that the control was handled with same process as samples.[Result] The maximum wavelength was confirmed.The error due to wet hot decomposing of the sample during the determination course was eliminated.[Conclusion] By the methodology inspection,the method. |
| Key words: Rehum Total anthraquinone Visible-spectrophotometric λmax Decompose |
