针刺对急性脑出血模型鼠微血管形态学的影响

Effect of Acupuncture on Morphology of Microvasculature in Rats with Acute Cerebral Hemorrhage

关键词:  针刺  急性脑出血模型鼠  微血管形态学  图形分析

Key words:  acupuncture  rats with acute cerebral hemorrhage  microvascular morphology  picture-analysis

摘要:

[目的]从微血管角度揭示针刺治疗急性脑出血的作用机制。[方法]从心脏以恒压灌入碳素墨水与甲醛混合液,显示血肿区周围微血管的形态,使用光镜观测与图形分析。[结果]脑出血后,可见血肿区周围充盈显影的血管数目减少,以一级血管显影为主,血管面积总和急剧下降,对照组各时相充盈显影血管总数目和显影血管面积总和均有提高,与模型组各时相相比,均具有显著性差异(P<0.05)。经针刺治疗处理后,血肿周围区满布一级血管及分支血管,有些已逆行伸入到血肿区,效果明显优于对照组。[结论]血肿区内血管及其周围区血管的增多,可为血肿区提供纤溶活性物质,促使血肿的进一步溶解,这可能是针刺治疗脑出血促进血肿吸收的途径之一。

Abstract:

[Objective] To explore the therapeutic mechanism of acupuncture in treating acute cerebral hemorrhage from the point of view of change of microvasculature. [Methods] The morphologic change of perihematoma microvasculature exhib-ited by cardiac pressure-constant perfusion with the mixture of carbon ink and formaldehyde was observed with optical microscopy and picture-analyzing system. [Results] After cerebral hemorrhage the number of exhibited vessels in perihe-matoma was declined.Most of them is referable to first grade.The total vascular area decreased dramatically.Both the total number of exhibited vessels and the area were increased in control group and had statistical significant difference compared with those in each phase in model group(P<0.05).After acupuncture first grade vessels were interspersed evenly in the perihematoma area with some protruding into the hematoma.The effect was markedly superior to that in control. [Conclusion] The increasing of blood vessels in the hematoma and the peripheral area can supply fibrinolytic substances on the hematoma to accelerate further lysis of it.That may be one of the ways of acupuncture in treating cerebral hemorrhage and facilitating the absorption of hematoma.