| 摘要: |
| [目的]探讨丹酚酸B(SalB)预适应的细胞保护机制是否与诱导热休克蛋白70(HSP70)合成有关。[方法]在缺氧复氧损伤大鼠心脏微血管内皮细胞(CMEC)后,采用RT-PCR法观察SalB预适应对细胞HSP70mRNA表达的影响。[结果]HSP70mRNA在正常的CMEC有较低表达,缺氧刺激使其表达明显增强。SalB预适应促进HSP70mRNA表达增强,作用明显优于缺氧预适应(HPC)。晚期预适应保护中,SalB预适应同样促使HSP70mRNA表达明显增强,与HPC具有增强表达的叠加效应。[结论]SalB预适应通过诱导HSP70表达升高发挥细胞保护作用。 |
| 关键词: 丹酚酸B 预适应 热休克蛋白 缺氧 复氧 |
| DOI：10.11656/j.issn.1672-1519.2004.06.06 |
| 分类号: |
| 基金项目:天津市自然科学基金资助项目(013612711) |
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| Effect of Salvianolic Acid B Preconditioning on Expression of Heat Shock Protein 70mRNA in Cardiac Microvascular Endothelial Cells Injured by Hypoxia and Reoxygenation |
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GUO Li-ping,ZHANG Meng,DU Rong
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The First Hospital affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To explore whether the mechanism of the cellular protection of salvisnolic acid B (Sal B) preconditioning is relevant with the synthesis of the heat shock protein 70 (HSP70). [Methods] Technique of RT-PCR was adopted,and the impact of Sal B preconditioning on the expression of heat shock protein 70 (HSP70) mRNA in cardiac microvascular endothelial cells (CMEC) injured by hypoxia and reoxygenation was observed. [Results] HSP70 produced less expression in normal CMEC and its expression was increased significantly by hypoxia stimulating. Sal B preconditioning enhanced the expression of HSP70 mRNA and the effect was obviously superior to that of hypoxic preconditioning (HPC). During the delayed preconditioning protection, Sal B also could increase the HSP70 mRNA expression and enhance the effect of HPC. [Conclusion] Sal B preconditioning produces a protective effect on cells by inducing the expression of HSP70. |
| Key words: salvianolic acid B preconditiong heat shock protein70 hypoxia and reoxygenation |
