|
| 摘要: |
| [目的]研究冰片对大鼠肝脏细胞色素氧化酶(cytochromeP450,CYP450)含量及其亚型CYP3A1m RNA表达的影响。[方法]istar大鼠合成冰片(设0.3gk/g和0.03gk/g剂量组,1次d/,连续7d)灌胃,以溶媒羧甲基纤维素纳(CM C-Na)和苯巴比妥钠为对照组,钙沉积法提取肝微粒体,紫外分光光度法测定CYP450含量,实时定量RT-PCR法检测肝脏CYP3A1m RNA的表达。[结果]0.3gk/g合成冰片口服可诱导大鼠肝脏CYP450酶含量增高(P<0.05),CYP3A1m RNA表达上调(P<0.05)。[结论]高剂量合成冰片口服可能影响肝脏药物代谢。 |
| 关键词: 合成冰片 肝脏CYP450酶 CYP3A1 药物代谢 |
| DOI：10.11656/j.issn.1672-1519.2005.04.08 |
| 分类号: |
| 基金项目:国家重点基础研究发展项目(973)(200C5B523404);天津市自然科学基金课题(013613711) |
|
| Impact of Borneolum Syntheticum on Contents of YP450 in Rat Liver and Expression of CYP3A1 mRNA |
|
HU Li-min, JIANG Min, WANG Shao-xia
|
| Abstract: |
| [Objective] To observe the impact of Borneolum Syntheticum on the cytochrome oxidase P450(CYP450) of liver and the expression of its subgroup CYPA3 mRNA in rat.[Methods] Borneolum Syntheticum was given orally to Wistar rats for 7days.Liver microsome was separated with calcium carbonate precipitation and CYP450 enzyme content was measured with ultraviolet spectrophotometry.The expression of CYP3A1 mRNA was detected with Real-time quantitative RT-PCR.[Results] 0.3 g/kg but 0.03 g/kg of Synthetic Borneol could increase the content of CYP450 enzyme in rat liver(P<0.05),and upregulate the expression of the CYP3A1 mRNA(P<0.05).[Conclusion] High dose of Synthetic Borneol may affect the metabolism of medicine in liver. |
| Key words: Borneolum Syntheticum cytochrome oxidase P450 CYP3A1 mRNA metabolism of medicine |
