毓宫胶囊对肾阳虚型子宫发育不良大鼠IL2-IFN-NKC免疫调节网影响的研究

Influence of Yugong Capsule on IL-2-IFN-NKC Immunological Regulation Network in Rats with Deficiency of Kidney-Yang Syndrome and Dysplasia of Uterus

关键词:  毓宫胶囊  肾阳虚  子宫发育不良  免疫调节  实验研究

Key words:  Yugong Capsule  deficiency of kidney-yang  dysplasia of uterus  immunological regulation  experimental research

摘要:

[目的]观察毓宫胶囊对肾阳虚型子宫发育不良大鼠IL₂(白介素-2)-IFN(干扰素)-NKC(自然杀伤细胞)免疫调节网的影响,探讨其治疗子宫发育不良的机制。[方法]通过对幼龄SD雌鼠40只随机分为4组:正常对照组(A组)、模型组(B组)、右归丸组(C组)、毓宫胶囊组(D组),肌注地塞米松复合双侧卵巢切除建立肾阳虚型子宫发育不良模型,对模型动物用毓宫胶囊治疗,右归丸做对照,治疗18d后处死,取胸腺、脾脏、子宫称质量,并测定脾组织IL₂、IFN、NKC活性。[结果]肾阳虚子宫发育不良大鼠胸腺、脾脏、子宫质量以及IL₂、IFN、NKC活性均较正常组明显下降(P<0.01),毓宫胶囊能提高胸腺、脾脏、子宫质量及IL₂、IFN、NKC活性(P<0.01)。[结论]肾阳虚型子宫发育不良大鼠的非特异性免疫力下降;毓宫胶囊在促进肾阳虚型子宫发育不良大鼠子宫发育的同时,提高IL₂、IFN、NKC活性,正向调节其免疫功能。即毓宫胶囊有调节神经内分泌免疫网的作用。

Abstract:

[Objective] To observe the influence of Yugong(invigorating the uterus)Capsule on IL₂-IFN-NKC immunological regulation network in rats with deficiency of kidney-yang syndrome and dysplasia of uterus and discuss the mechanism of treating dysplasia of uterus.[Methods] Forty immature SD female mice were randomly divided into four groups:control group(A),model group(B),Yougui pills group(C),Yugong capsule(D).The deficiency kidney-yang syndrome and dysplasia of uterus in rats were established by injecting hexadecadrol and bilateral oophorectomy.The weight of thymus,spleen,uterus,the IL-2,IFN and NKC activity of the spleen were determined.[Result] The weight of thymus,spleen uterus and IL-2,IFN and NKC activity in the treatment group were obviously lower than that in the normal group in rays with deficiency of kidney-yang syndrome and dysplasia of uterus(P<0.01).Yugong Capsule could increase the weight of thymus,spleen,uterus and IL-2,IFN and NKC activity(P<0.01).[Conclusion] Nonspecific immunity decreased in rats with deficiency of kidney-yang syndrome and hypoplasia of uterus.Yugong Capsule can promote the development of uterus,enhance IL-2,IFN and NKC activity and up-regulate its immune function in rats with deficiency of kidney-yang syndrome and sysplasia of uterus.YuGong capsule possesses a regulative effect on nerve-endocrine-immune networks.