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HPLC法同时测定香加皮中杠柳毒苷和4-甲氧基水杨醛
刘虹, 杨虹, 孙巍, 王萌, 郭俊华, 潘桂湘
天津中医学院, 天津, 300193
摘要:
[目的]同时测定香加皮中杠柳毒苷和4-甲氧基水杨醛的含量。[方法]采用HPLC法,ODS色谱柱,流动相为乙腈:水=27:73,检测波长220nm。[结果]杠柳毒苷线性范围0.05~1.6μg,r=0.9994;4-甲氧基水杨醛线性范围0.23~7.4μg,r=0.9993;杠柳毒苷和4-甲氧基水杨醛的精密度分别为1.2%、3.0%,重现性分别为1.4%、2.8%,加样回收率分别为100.2%、99.8%。[结论]本法简便、准确,可同时测定香加皮中的杠柳毒苷和4-甲氧基水杨醛。
关键词:  香加皮  杠柳毒苷  4-甲氧基水杨醛  HPLC
DOI:10.11656/j.issn.1672-1519.2006.01.25
分类号:
基金项目:天津市高等学校科技发展基金项目(20050310);天津市卫生局中医、中西医结合科研基金(2005072);天津市科委应用基础研究计划项目(05YFJZJC01102)
Simultaneous Determination of Periplocin and 4-Methoxysalicylic Aldehyde in Cortex Periplocae with HPLC
LIU Hong, YANG Hong, SUN Wei
Tianjin University of TCM, Tianjin 300193, China
Abstract:
[Objective] To determine the content of Periplocin and 4-methoxysalicylic aldehyde in Cortex Periplocae simultaneously.[Methods] The determination was carried out by HPLC on ODS chromatographic column,using acetonitrile-water(27:73)as the mobile phase at 220 nm.[Result] The calibration curve was linear within the range of 0.05~1.6 μg(r=0.999 4)for periplocin and 0.23~7.4 μg(r=0.999 3)for 4-methoxysalicyl aldehyde.The precision of the two constituents was 1.3% and 2.5%,the reproducibility was 1.4% and 2.8% and the spotting recovery rate was 100.2% and 99.8% respectively.[Conclusion] This method is convenient and accurate.It can be used to determine periplocin and 4-methoxysalicylic aldehyde simultaneously.
Key words:  Cortex Periplocae  periplocin  4-methoxysalicylic aldehyde  HPLC
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