活血化瘀注射液I号对大鼠高血压性血-脑屏障破坏的保护作用

Protective Effect of HHI-I Against Phenylephrine-Induced Blood-Brain Barrier Permeability Breakdown in Rats

关键词:  活血化瘀注身液I号  高血压  血脑屏障  实验研究

Key words:  HHI-I  hypertention  blood-brain barrier  experimental research

摘要:

[目的]探讨活血化瘀注射液I号(HHI-I)对大鼠高血压性血-脑屏障破坏的保护作用。[方法]将大鼠随机分为6组,10只/组:空白对照组(不作任何处理),高血压模型组(股静脉内注射苯肾上腺素后不治疗),低、中、高剂量给药组(静脉内注射1.5%、3.0%、6.0%浓度的HHI-I,2mL/kg,和治疗对照组(以生理盐水代替HHI-I)。各组动物按设计分组处理,60min后股静脉内注射伊文思蓝,再5min后给予苯肾上腺素,30min后处死动物取脑组织标本,测定伊文思蓝(EB)含量。[结果]模型组血-脑通透性明显增加,与正常组比较P<0.05;生理盐水未使增高的通透性降低,而3个剂量的HHI-I均能使通透性明显降低,P均<0.05。[结论]HHI-I对大鼠高血压性血-脑通透性破坏有明显保护作用。

Abstract:

[Objective] To investigate the protective effect of HHI-I against the blood-brain barrier(BBB)permeability breakdown induced by phenylephrine in hypertensive rats.[Methods] Sixty male Wistar rats,weighing about 220 g were randomly divided into 6 groups with 10 animals in each.While the blank control group and model group were treated with nothing,three treating groups were received low,moderate and high dose of HHI-I(1.5%,3% and 6%,2 mL/kg respectively).The control group didn’t receive any treatment but equivalent normal saline.One hour after such intervention all these groups were intravenously injected with the indicator Evans blue,2%,4 mg/kg,b.w.Five minutes later the hypertension was induced in the last five groups with phenylephrine,0.01%,1.5 mg/kg,b.w.to result in BBB breakdown.Thirty minutes after that all the rats were killed and the cortex of hemisphere was harvested to measure the concentration of Evans blue in it.[Results] The BBB permeability was obviously increased in the rats of model group compared with the blank control(P<0.05).No change of BBB was observed in the N.S.control group.In contrast with those in model group,all the three doses of HHI-I could markedly decline the increased BBB permeability induced by phynylephrine.[Conclusion] HHI-I has significant protective effect against BBB permeability breakdown in hypertensive rats.