活血化瘀中药对清热利胆中药利胆作用的增效研究

Synergic Action of Chinese Herbs with HuoxueHuayu Effect on Cholagogic Effect of Chinese herbs with QingreLidan Effect

关键词:  活血化瘀中药  清热利胆中药  利胆灵  增效  大鼠“T”管胆肠内外引流模型

Key words:  HuoxueHuayu herbs  QingreLidan herbs  Lidanling  synergistic action  rat  T-tube for external drainage of bile

摘要:

[目的]探讨活血化瘀中药对清热利胆中药利胆的增效作用。[方法]选用健康Wistar大鼠36只,随机分为6组:正常组、生理盐水对照组、活血化瘀组、清热利胆组、利胆灵组(活血化瘀+清热利胆)、熊去氧胆酸组,每组6只。除正常组外,其余5组建立大鼠“T”管胆肠内外引流模型。各组大鼠给药7d后收集标本,分别测定各组大鼠胆汁流量、胆汁及血清胆红素、直接胆红素、总胆汁酸、胆固醇、磷脂。[结果]与正常组、生理盐水对照组、活血化瘀组及清热利胆组相比,熊去氧胆酸组与利胆灵组均能显著促进正常大鼠胆汁流量、胆汁胆红素、胆汁总胆汁酸的分泌,降低胆汁胆固醇的含量;能显著降低血清胆红素、血清总胆汁酸、血清胆固醇水平;对胆汁及血清磷脂水平影响不明显。[结论]活血化瘀中药对清热利胆中药有增效作用。

Abstract:

[Objective] To investigate the synergistic action of HuoxueHuayu(promoting blood circulation and dissolving stagnant blood)herbs on the cholagogic effect of QingreLidan medicine(clearing away heat and promoting bile secretion)herbs.[Methods] Thirty-six healthy Wistar rats were randomly divided into six groups:normal group,saline control group,HuoxueHuayu group,QingreLidan group,Lidanling(HuoxueHuayu and QingreLidan herbs together)group and Ursodeoxycholic acid group.Except for the normal group,a T-tube for external drainage of bile was inserted in all the other groups.Specimens were collected seven days after administration to determine individually bile flow rate,bile and serum bilirubin,direct bilirubin,total bile acid,cholesterol and phospholipids.[Result] Compared with other groups,both ursodeoxycholic acid group and Lidanling group may significantly promote normal rat bile flow rate,bile bilirubin,total bile acid and decreased bile cholesterol,significantly decreased serum bilirubin,serum total bile acid and serum cholesterol levels with little effect on phospholipid of bile and serum.[Conclusion] HuoxueHuayu herbs has showed synergistic action with QingreLidan herbs.