洋参二醇皂苷注射液对大鼠心肌缺血再灌注损伤的影响

Effect of IPQDS on Myocardial Ischemia and Reperfusion Injury in Rats

关键词:  IPQDS  心肌缺血再灌注损伤  血清酶  自由基  血管活性因子

Key words:  IPQDS  myocardial ischemia and reperfusion injury  serum enzyme  free radicals  vasoactive factors

摘要:

[目的]研究洋参二醇皂苷注射液(IPQDS)对大鼠心肌缺血再灌注损伤的保护作用。[方法]在体大鼠结扎冠状动脉前降支30min后,松扎再灌注120min造成心肌缺血再灌注模型,计算心肌梗死范围(MIS),测定血清磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)、天门冬氨酸氨基转换酶(AST)、超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及脂质过氧化物(LPO)含量,血浆内皮素(ET)、血管紧张素Ⅱ(AngⅡ)、前列环素(PGI₂)及血栓素A₂(TXA₂)水平。[结果]IPQDS对心肌缺血30min再灌注损伤120min大鼠,可明显缩小MIS,降低血清CK、LDH、AST活性及LPO含量,提高SOD及GSH-Px活性,能使血浆ET、AngⅡ、TXA₂水平明显下降,PGI₂水平及PGI₂/TXA₂比值明显增高。[结论]IPQDS对大鼠心肌缺血再灌注损伤具有明显保护作用,可能与其增强抗氧化酶活性,减少自由基对心肌的氧化损伤,减少内源性血管活性物质ET及AngⅡ释放,纠正PGI₂/TXA₂失衡等机制有关。

Abstract:

[Objective] To observe the effect of Injection of Panax quinquefolii radix 20s-protopanaxdiol saponins (IPQDS) on rats with myocardial ischemia and reperfusion injury.[Methods] The myocardial ischemia and reperfusion injury was induced by 30 min occlusion of the left anterior descending coronary artery and 120 min reperfusion in rats. The changes of myocardial infarcted size(MIS), serum creatine phosphokinase(CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) activity, serum lipid peroxidation (LPO) content, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, plasma endothelin (ET), angiotensinII(AngII), prostacycline (PGI₂) and thromboxane A₂(TXA₂) levels were determined.[Results] In rats treated with IPQDS at a dosage of 12.5, 25 and 50 mg/kg, infused at 30 min after coronary occulusion intravenously, the MIS was significantly reduced, the serum CK, LDH and AST activity and PLO were decreased, the plasma ET, AngII and TXA₂ level declined also, while plasma PGI₂ level and PGI₂/TXA₂, SOD and GSH-Px activity were increased significantly.[Conclusions] IPQDS has obvious protective effect on myocardial ischemia and reperfusion injury, probably by improving free radicals metabolism, decreasing plasma ET, AngⅡ and TXA₂ levels and increasing plasma PGI₂ level and PGI₂/TXA₂ ratio etc.