梁金菇多糖对人脐血单个核细胞生成白介素-2的影响

Effects of Liang Jin Mushroom Polysaccharide on IL-2 Generation in Mononuclear Cells of Human Umbilical Blood

关键词:  梁金菇多糖  脐血单个核细胞  IL-2  IL-2mRNA

Key words:  LiangJin Mushroom polysaccharide  umbilical blood mononuclear cells  interleukin-2  interleukin-2 mRNA

摘要:

[目的]研究梁金菇多糖(LJPS)对脐血单个核细胞(CBMC)分泌白细胞介素-2(IL-2)和IL-2mRNA表达的影响,探讨梁金菇多糖的增强免疫和抗肿瘤活性,为中国栽培的梁金菇药用价值的开发提供实验依据。[方法]采用放射性免疫法检测LJPS对CBMC分泌IL-2的影响,并应用RT-PCR法检测LJPS对CBMCIL-2mRNA表达的影响。[结果]LJPS单独或与植物血凝素(PHA)联合应用均能够促进CBMC分泌内源性IL-2(P<0.05和P<0.01),分泌高峰为48h左右,并且两者均能促进CBMCIL-2mRNA的表达,表达高峰为24h左右。[结论]梁金菇多糖能够促进CBMC内源性IL-2的分泌及IL-2mRNA的表达。

Abstract:

[Objective] To study the effects of Liangjin mushroom polysaccharide (LJPS) on the secretion of IL-2 and the expression of IL-2 mRNA from the mononuclear cells(CBMC) of human umbilical blood. To explore the immunologic and anti-tumor activity of LJPS and provide the experimental evidence for medical use of LiangJin Mushroom cultivated in China.[Methods] The secretion of IL-2 from CBMC was determined by radioimmunoassay method and the expression of IL-2 mRNA was detected by the reverse transcription polymerase chain reaction (RT-PCR).[Result] Both LJPS alone or combined with PHA could enhance the secretion of IL-2 from CBMC(P<0.05 and P<0.01). The peak was at about 48th hour later. Moreover, they could promote the expression of IL-2 mRNA in CBMC with the peak at about 24th hour.[Conclusion] LJPS can enhance the secretion of the endogenous IL-2 and the expression of IL-2 mRNA of CBMC.