针刺对慢性疲劳大鼠血清IL-2及TNF-α的影响

Influence of Acupuncture on Serum IL-2 and TNF-α in Rats with Chronic Fatigue Syndrome

关键词:  针刺  慢性疲劳综合征  白细胞介素-2  肿瘤坏死因子-α

Key words:  acupuncture  chronic fatigue syndrome  IL-2  TNF-α

摘要:

[目的]观察针刺对慢性疲劳大鼠血清白细胞介素-2(IL-2)及肿瘤坏死因子-α(TNF-α)的影响。[方法]采用慢性束缚和冷水游泳制备慢性疲劳大鼠模型。随机将大鼠分为正常对照组、模型组、针刺组和人参皂苷组。针刺组选百会、肾俞、后三里穴,针刺1次/d。人参皂苷组给予人参皂苷水溶液[70mg/(kg·d)]灌胃治疗,1次/d。正常对照组和模型组进行与针刺组和人参皂苷组相同时间、相同程度的捉抓。连续治疗2周断头取血。采用放射免疫分析法检测大鼠血清IL-2及TNF-α的水平。[结果]模型组大鼠血清IL-2、TNF-α水平均降低,针刺后两者均明显升高。[结论]针刺可以升高慢性疲劳大鼠血清IL-2、TNF-α水平,这可能是针刺治疗慢性疲劳综合征的作用机制之一。

Abstract:

[Objective] To observe the influence of acupuncture on the level of serum IL-2 and TNF-α in rats with chronic fatigue syndrome(CFS). [Methods] The CFS rats model was established by chronic bondage and swimming in cool water. The selected rats were randomly divided into four groups: normal group, model group, acupuncture group and panaxoside group. In acupuncture group, Baihui point, Shenshu point and Housanli point were selected. The acupuncture point was pricked once a day. In panaxoside group, panaxoside solution (70 mg/(kg·d)) was infused into the stomach of rats once a day. The rats in normal group and model group were only caught and grasped at the same time interval and degree with the treatment group. Two weeks after treatment, the rats were killed by head-dislocation and serum was harvested. The level of serum IL-2 and TNF-α was determined by radio-immunological analysis. [Results] The level of serum IL-2 and TNF-α was significantly decreased, and it was obviously increased after acupuncture treatment. [Conclusions] The acupuncture may increase the level of serum IL-2 and TNF-α in rats with CFS. This action may be one of the mechanisms of acupuncture in treating CFS.