针刺对局灶性脑梗死大鼠软脑膜微血管自律运动的影响

Effect of Acupuncture on Automatic Rhythmicity in Microvasculature of Cerebral Pia Mater in Rats with Local Infarction

关键词:  脑梗死  针刺  脑微循环  自律运动

Key words:  cerebral infarction  acupuncture  automatic rhythmicity of cerebral microcirculation

摘要:

[目的]观察针刺对脑梗死大鼠软脑膜微血管自律运动的影响。[方法]以大脑中动脉阻断(MCAO)大鼠为模型,采用血管内皮细胞荧光染色法,结合显微录相装置和计算机图象分析系统,动态定量地观察针刺对MCAO后3、6、24h软脑膜微血管自律运动的频率、振幅的影响。[结果]MCAO后3、6、24h,缺血区软脑膜微血管自律运动紊乱,表现为高频率、低振幅的运动。针刺组频率明显降低,振幅有所增高。[结论]针刺对改善脑灌流有重要意义。

Abstract:

[Objective] To observe the effect of acupuncture on the automatic rhythmicity in microvasculature of cerebral pia mater in rats with local infarction. [Methods] The model of local brain infarction with middle cerebral artery occlusion(MCAO) was used and the method of fluorescence staining of vascular endothelial cells was applied. With videomicroscopy and image-analyzed system of computer the effect of acupuncture on the automatic rhythmicity of microvasculature, including frequency and amplitude, in cerebral pia mater was observed dynamically 3, 6 and 24 hours after MCAO. [Results] 3, 6, 24 hours after MACO, a disturbance of the automatic rhythmicity in cerebral pia mater of ischemia region, appeared as high frequency and low amplitude, was observed. In the acupuncture group, however, the frequency decreased obviously and the amplitude increased in a certain degree. [Conclusions] Acupuncture can improve the cerebral microcirculation.