益肾达络饮对实验性自身免疫性脑脊髓炎MCP-1的影响

Effect of Yinshendaluoyin on MCP-1 of Mice with Experimental Autoimmune Encephalomyelitis

关键词:  自身免疫性脑脊髓炎  多发性硬化  益肾达络饮  髓鞘蛋白脂质蛋白多肽

Key words:  autoimmune encephalomyelitis  multiple sclerosis  Yishendaluoyin  MCP-1

摘要:

[目的] 研究益肾达络饮对髓鞘蛋白脂质蛋白多肽(PLP139-151)诱导的实验性自身免疫性脑脊髓炎小鼠中枢神经系统内趋化因子(MCP-1)的影响,探讨益肾达络饮在神经系统炎性脱髓鞘疾病中的作用机制。[方法] 采用免疫组化、地高辛标记的寡核苷酸探针原位杂交技术熏测定各组小鼠中枢神经组织中MCP-1蛋白和MCP-1mRNA的含量。[结果] 正常组与模型组、激素组、中药组MCP-1蛋白、MCP-1mRNA含量相比较均有极显著性差异(P<0.01),中药组、激素组与模型组MCP-1蛋白、MCP-1mRNA含量相比较均有极显著性差异(P<0.01);中药组、激素组相比较有显著性差异(P<0.05)。[结论] 益肾达络饮在蛋白、分子水平能降低MCP-1含量,其对自身免疫性脑脊髓炎小鼠保护作用可能与下调MCP-1熏抑制单个核细胞的趋化性熏减轻炎性浸润,抑制局部炎症有一定关系。

Abstract:

[Objective] To investigate the effect of Yishendaluo(tonifying kidney and dredging collateral) decoction on MCP-1,the chemotactic factor of central nervous system,in mice with PLP139-151-induced experimental autoimmune encephalomyelitis and explore the mechanism of Yishendaluo decoction on the inflammatory demyelinating diseases in the nervous system.[Methods] Female mice were evenly randomized into normal control,model,hormone and traditional Chinese medicine groups. The contents of MCP-1 protein and MCP-1 mRNA were detected by immunohistochemistry and in situ hybridization of digoxin-labeled oligonucleotide probe.[Results] The contents of MCP-1 protein and MCP-1 mRNA in model,hormone and traditional Chinese medicine groups were all significantly higher than those in normal control group (P<0.01). Compared with model group,the augmentation in contents of MCP-1 protein and MCP-1 mRNA in both hormone group and traditional Chinese medicine group was significantly diminished (P<0.01). In contrast to hormone group,the contents of MCP-1 protein and MCP-1 mRNA in traditional Chinese medicine group were decreased (P<0.05).[Conclusions] The results of the present study indicate that Yishendaluo decoction can protect the mouse from the injury of experimental utpommune encephalomyelitis by attenuating the content of MCP-1,inhibiting the chemotaxis of monocytes and lessening the local inflammation.