糖肾清Ⅰ号对糖尿病大鼠肾脏保护作用的实验研究

Protective Effect of Tangshenqing-1 on Kidney in Rats with Diabetic Nephropathy

关键词:  糖尿病肾病  糖肾清Ⅰ号  实验研究

Key words:  diabetic nephropathy  Tangshenqing-1  experiment

摘要:

[目的]探讨糖肾清I号对糖尿病肾病大鼠肾脏的保护作用。[方法]将四氧嘧啶所诱导的清洁级Wistar糖尿病肾病大鼠36只,随机分为正常对照组(A组)、糖尿病肾病未干预组(B组)和糖尿病肾病糖肾清Ⅰ号干预组(C组),每组各12只。糖尿病肾病模型成功后开始给予糖肾清Ⅰ号药物干预,8周后收集24h尿液及血标本,处死大鼠后,取肾组织行光镜、电镜、免疫组织化学及分子生物学检查。[结果]糖尿病肾病大鼠肾组织转化生长因子β1(TGF-β1)基因及蛋白水平表达明显增加,尿蛋白增加,肌酐清除率上升;糖肾清Ⅰ号使尿蛋白减少(P<0.01),肌酐清除率降低(P<0.01),TGF-β1基因及蛋白水平表达明显下降,病理学检查显示糖肾清Ⅰ号使糖尿病肾小球硬化减轻。[结论]糖肾清Ⅰ号通过部分抑制TGF-β1的活性而对糖尿病肾脏损害起到保护作用。

Abstract:

[Objective] To investigate the renal protective effect of Tangshenqing-1(treating diabetic nephropathy)in rats with diabetic nephropathy.[Methods] Thirty-six rats with intraperitoneal alloxan induced diabetic nephropathy were randomly divided into three groups with 12 animals in each:normal control(A),diabetic nephropathy without any treatment(B),and diabetic nephropathy treating with Tangshenqing-1(C).After the model was established Tangshenqing-1 was administered.Eight weeks later the 24 h urine and blood samples were collected.Then rats were killed.The kidneys were harvested for microscopic,electro-microscopic,immunohistochemistry and molecular biology examination.[Results] The TGF-b1 gene and protein expression,the creatinine clearance rate and urea protein in the kidney tissue of rats with diabetic nephropathy were significantly higher in group B and groupC than groupA(P<0.01).However,the degree of diabetic glomerular-sclerosis were decreased by treatment of Tangshenqing-1.[Conclusions] Tangshenqing-1 can protect diabetic kidney through deceasing the expression of TGF-b1.