黄芪注射液对高糖或AGEs培养肾小球系膜细胞增殖和Na+-K+-ATP酶活性的影响

Influence of Astragalus Injection on Proliferation and Na+-K+-ATPase Activity of Glomerular Mesangial Cells Cultured in High Glucose or AGEs Medium

关键词:  黄芪注射液  肾小球系膜细胞  细胞增殖  Na⁺-K⁺-ATP酶

Key words:  astragalus injection  glomerular mesangial cell  cell proliferation  Na⁺-K⁺-ATPase

摘要:

[目的]观察黄芪注射液对高糖或糖化终产物(AGEs)培养肾小球系膜细胞(GMC)增殖和Na⁺-K⁺-ATP酶活性的影响。[方法]用含高糖或AGEs的培养液配制黄芪注射液、氨基胍,同时设对照。GMC培养48h后,四甲基氮唑盐(MTT)法考察GMC的增殖情况,试剂盒测试细胞悬液中细胞蛋白含量,再进行Na⁺-K⁺-ATP酶测定。[结果]葡萄糖或AGEs中GMC的吸收度值增大,Na⁺-K⁺-ATP酶活性显著降低,与正常培养液比较具有显著性差异。而加入黄芪注射液后,则表现为对抗高糖或AGEs对GMC的增殖作用,并能提高Na⁺-K⁺-ATP酶活性。[结论]黄芪注射液能抑制高糖或AGEs对GMC的过度增殖,修复高糖或AGEs致GMC的Na⁺-K⁺-ATP酶活性降低,起到保护细胞的作用。

Abstract:

[Objective] To observe the influence of astragalus injection on the proliferation and Na⁺-K⁺-ATPase activity of glomerular mesangial cells(GMC)cultured in high glucose or glycosylation end products(AGEs)medium.[Methods] GMC were incubated in culture medium containing high glucose or AGEs in the presence of astragalus injection and aminoguanidin for 48 hours.At the same time,the normal and control groups were added.The proliferation of GMC were analyzed by MTT method.The Na⁺-K⁺-ATPase activity were tested by Na⁺-K⁺-ATPase test Kit.[Results] GMC proliferated remarkably in glucose or AGEs medium in comparison with that in 5% FBS RPMI-1640 culture medium,and the Na⁺-K⁺-ATPase activity remarkably decreased.While the astragalus injection were added,the excess proliferation of GMC can be mostly reversed,and the Na⁺-K⁺-ATPase activity showed a significant restoration in comparison with that in control groups.[Conclusions] Astragalus injection can inhibit the excess proliferation and restore the decreased Na⁺-K⁺-ATPase activity of GMC induced by high glucose or AGEs,so it can protect the GMC.