丹参注射液对严重烫伤大鼠血清细胞因子含量的影响

Effect of Danshen injection on serum cytokine content in severe burned rats

关键词:  丹参注射液  烫伤  白细胞介素-6  白细胞介素-10

Key words:  Danshen injection  burn  interleukin-6  interleukin-10

摘要:

[目的] 观察大鼠严重烫伤早期血清前炎性细胞因子白细胞介素-6(IL-6)和抗炎细胞因子白细胞介素-10(IL-10)的动态变化及丹参注射液对它们的影响。[方法] 建立30%Ⅲ度烫伤模型,88只Wistar大鼠随机分为正常对照组(A组,8只)、平衡液复苏组(B组,40只)和丹参治疗组(C组,40只)。B、C两组烫伤后立即按Parkland公式腹腔注射平衡液120mL/kg,C组在同样治疗的基础上腹腔注射丹参注射液3mL/kg,B、C两组分别于伤后3、6、12、24、48h检测血清IL-6和IL-10的含量。[结果] 与A组比较,B组血清IL-6的含量在伤后3h即明显高于A组,伤后12h达高峰;B组血清IL-10的含量在伤后6h即明显高于A组,伤后24h达高峰;C组在各相应时相点与对应的B组比较,两者的变化趋势基本一致。[结论] 严重烫伤早期血清IL-6、IL-10即明显升高;丹参注射液可通过降低血清IL-6提高血清IL-10而发挥抗炎作用。

Abstract:

[Objective] To observe the dynamical change of serum proinflammatory interleukin-6 (IL-6) and antiinflammatory interleukin-10 (IL-10) level and the impact of Danshen(miltiorrhiza,SM) injection on them in early stage of severe burn in rats.[Methods] Eighty-eight Wistar rats were randomly divided into three groups: normal control group(group A),burn group (group B),and burn plus SM group(group C).Rats of group B and C received a 30%,Ⅲ-degree burn and were resuscitated immediately with lactated Ringer’s solution (120mL/kg).Additionally,rats of group C received Danshen injection at a dose of 3mL/kg.The serum content of IL-6 and IL-10 were measured 3,6,12,24 and 48h after burn.[Results] In group B,serum IL-6 increased remarkably 3h after burn and reached the peak at 12 h post-burning; serum IL-10 increased remarkably 6 h after burn and reached the peak at 24h post-burn; The change tendency of above index in group C was similar to that in group B.[Conclusions] The serum content of IL-6,IL-10 increased immediately in severely burned rats at earlier period,Danshen injection has anti-inflammatory effect by decreasing the level of serum IL-6 and enhancing the level of serum IL-10.