金莲花蛋白超声提取工艺优化及其抑菌活性的初步测定

Optimization of process for ultrasonic extraction and primary determination of bacteriostatic activity of protein from flos trollii chinensis

关键词:  金莲花蛋白  抑菌活性  提取工艺

Key words:  protein from Flos Trollii Chinensis  restrain bacterium action  extraction technical

摘要:

[目的] 优化金莲花(Flos Trollii Chinensis)蛋白超声提取工艺,并测定蛋白提取液的抑菌活性。[方法] 用L9(34)正交设计优化超声提取金莲花蛋白工艺,用纸片扩散法检测金莲花蛋白抑菌活性。[结果] 金莲花蛋白超声提取的最佳工艺为:原料粒度60目,料液比1:30,提取时间30min。金莲花蛋白提取液(1.25g/L)具有抑制变形杆菌(++)、伤寒杆菌(++)、产气杆菌(+)和大肠杆菌(+)的作用。[结论] 利用超声提取方法能够获得高产率的金莲花蛋白,并能保持其生物活性。

Abstract:

[Objective] To optimize the ultrasonic extraction technology for the protein from Flos Trollii Chinensis and determinate the bacteriostatic activity of the protein extract.[Methods] By applThe L9(34) orthogonal test was applied to optimize the ultrasonic extraction technology and the activity of bacteriostasis was determined with slip diffusion method.[Results] The optimized ultrasonic extraction technology was as follows: material granule 60 order,proportion of material to solution 1:30,extracted time 30 min.The extracted protein at 1.25 g/L could inhibit Bacillus proteus(++),Bacillus typhosus(++),Bacillus aerogenes(+),Bacterium coli(+).[Conclusions] The method with ultrasonic extraction can obtain higher plurinatality of protein of Flos Trollii Chinensis without any loss of its biologic activity.