紫外分光光度法测定炒决明子中总蒽醌含量

Content analysis of Anthraquinones in stir-baked semen cassiae by ultraviolet Spectrophotometry

关键词:  决明子  小决明子  总蒽醌  紫外分光光度法

Key words:  semen cassiae  Cassia tora  anthraquinones  ultraviolet spectrophotometry

摘要:

[目的]测定炒决明子中总蒽醌的含量,建立决明子中总蒽醌的快速定量测定方法,同时对不同产地的生、炒决明子中总蒽醌成分的含量进行测定。[方法]采用紫外分光光度法,检测波长为513nm。[结果]炒决明子中的总蒽醌在0.00246～0.01968g/L范围内与吸收度线性关系良好(r=0.9999)。加样回收率:平均回收率为96.9738%,峰面积(RSD)=1.0545%。[结论]该方法简单易行,重现性好,结果可靠,可用于决明子中总蒽醌的含量测定。

Abstract:

[Objective] To analyze anthraquinones contents in the stir-baked semen cassiae and find out a quantitative determination method for examining the effective content of anthraquinones in it. Simultaneously, the contents of anthraquinones in the crude and stir-baked ones from different districts are compared. [Methods] The ultraviolet spectrophotometry was used and the determination was performed at the wavelength of 513 nm. [Results] The linear relationship was fine(r=0.999 9) in 0.002 46～0.019 68 mg/ml. The recovery rate of anthraquinones was 96.973 8%, RSD=1.054 5%. [Conclusions] This method is simple and accurate. It can be used to detect the content of anthraquinones in the stir-baked semen cassiae.