蒲黄及炒蒲黄指纹图谱的初步研究

HPLC fingerprint analysis of Pollen Typhae and Pollen Typhae Parch

关键词:  蒲黄  炒蒲黄  指纹图谱

Key words:  Pollen Typhae  Pollen Typhae Parch  HPLC-FPS

摘要:

[目的]采用高效液相色谱法建立蒲黄、炒蒲黄的指纹图谱,并进行分析比较。[方法]WatersX-TerraC18(4.6mm×25cm)色谱分析柱,乙腈-水梯度洗脱,流速1.0mL/min,检测波长254nm。[结果]初步建立了蒲黄、炒蒲黄的指纹图谱,其精密度,重复性,稳定性实验中共有峰相对保留时间和相对峰面积(RSD)均小于3%。[结论]该方法简便、准确、重复性好,蒲黄炒制前后指纹图谱有明显差异。

Abstract:

[Objective] Reversed high-performance liquid chromatography (PR-HPLC) fingerprint of Pollen Typhae and Pollen Typhae Parch was established and analyzed. [Methods] Chromatographic conditions were available as follows: Waters X-Terra C18(4.6 mm×25 mm), ACN and water as mobile phase, gradient elution, flow rate 1.0 ml/min, the detection wavelength was set to 254 nm. [Results] We tried to establish the introduction of the fingerprint of Pollen Typhae of field thistle and Pollen Typhae Parch of field thistle. The RSD of relative peak areas and retention time of common peak in precision, reproducibility, and stability were not reached 3.0%. [Conclusions] All the above results exhibits that this method is simple, accurate, and reproducible and can be a standard in controlling the quality. There is a great difference in fingerprint after parched.