混合絮凝剂纯化淫羊藿醇提液工艺的研究

Optimizing the purification process of herba epimedii by mixed flocculation agents

关键词:  淫羊藿  絮凝剂  醇提水沉  纯化

Key words:  herba epimedii  mixed flocculation agent  lcohol-extraction and water precipitation  purification

摘要:

[目的]优选利用混合絮凝剂纯化淫羊藿醇提液的工艺。[方法]以淫羊藿苷的损失率以及鞣质、叶绿素的残留率为指标,采用综合加权评分法,对明胶、壳聚糖的加入量进行考察,并与传统水沉工艺进行比较。[结果]采用混合絮凝剂,分别加入3%药液量的明胶溶液和4%药液量的壳聚糖溶液,效果最好,淫羊藿苷的损失率仅为7.5%,鞣质和叶绿素的去除率分别达到56.3%和98.2%。[结论]添加混合絮凝剂用于中药醇提液的纯化具有良好的应用前景。

Abstract:

[Objective] To optimize the purification process of Herba epimedii by mixed flocculation agents. [Methods] The addition volume of flocculation agents were investigated and compared with traditional water-precipitation process using the yield loss rate of Icariin and residue percentage of tannin and chlorophyll as index by comprehensively weighted grading method. [Results] The optimum process was achieved when adding 3% extraction volume of gelatin and 4% extraction volume of Chitosan. The yield loss rate of Icariin was only 7.5% while the removal rate of tannin and chlorophyll reached 56.3% and 98.2%, respectively. [Conclusions] The method of adding mixed flocculation agents was promising in the field of refining alcohol-extraction of Chinese herbal medicines.