心脑血脉宁对家兔易损斑块模型血清CRP和斑块内炎细胞浸润的影响

Effects of Xinnao Xuemai Ning on serum CRP and inflammatory cell infiltration in plaque in rabbit with vulnerable plaque

关键词:  易损斑块  血清CRP  炎细胞  心脑血脉宁

Key words:  vulnerable plaque  serum CRP  inflammatory cell  Xinnao Xuemai Ning

摘要:

[目的]探讨心脑血脉宁对家兔易损斑块内炎细胞浸润和血清C反应蛋白(CRP)的影响。[方法]通过球囊拉伤主动脉内皮配合高脂饲料喂养复制家兔易损斑块模型。术后1周始,给予心脑血脉宁口服12周,于处死前24h和48h,分别予以药物触发斑块破裂,触发前收集血液,应用酶联免疫吸附实验(ELISA)法检测家兔血清CRP含量,处死后切取斑块,观察炎细胞浸润情况。[结果]心脑血脉宁可降低血清CRP的浓度,减少斑块内炎细胞浸润,与模型组相比有显著性差异。[结论]心脑血脉宁可以通过抑制炎症反应,达到稳定易损斑块的目的。

Abstract:

[Objective] To observe the effect of Xinnao Xuemai Ning (calming heart and brain and blood vessel) on serum CRP and the infiltration of inflammatory cell in plaque of rabbit with vulnerable plaque. [Methods] The vulnerable atherosclerotic plaque model was reproduced by pulling injury of abdominal aorta endothelium combined with high lipid forage. One week later,Xinnao Xuemai Ning was administered to the vulnerable atherosclerotic plaque model for twelve weeks. At the end blood was collected for CRP test by ELISA,then the plaque was disrupted with medico-triggered at 48 h and 24 h before sacrificing the animals. Abdominal aorta plaques were gathered for observation of inflammatory cell infiltration.[Results] The positive correlation was found between the serum CRP and inflammatory cell infiltration in plaque. Xinnao Xuemai Ning could reduce the number of plaque rupture and the level of serum inflammatory CRP. There was significant difference between model group and Xinnao Xuemai Ning group(P<0.05).[Conclusions] Xinnao Xuemai Ning can increase the stability of vulnerable atherosclerotic plaque,Its mechanism of action was correlated with anti-inflammation.