RP-HPLC测定活血止痛片中三七皂苷R1和人参皂苷Rg1含量

Quantitative determination of notoginsenoside R1 and ginsenoside Rg1 in Huoxue Zhitong tablet by RP-HPLC method

关键词:  活血止痛片  反相高效液相色谱法  三七皂苷R₁  人参皂苷Rg₁

Key words:  Huoxue Zhitong tablet  RP-HPLC  Notoginsenoside R₁  Ginsenoside Rg₁

摘要:

[目的]建立活血止痛片中三七皂苷R₁和人参皂苷Rg₁的含量测定方法。[方法]采用反相高效液相色谱法(RP-HPLC)。[结果]三七皂苷R₁的线性范围为0.207~1.036μg,人参皂苷Rg₁的线性范围为0.829~4.148μg,样品平均回收率分别为96.1%,97.3%,RSD分别为2.02%,2.34%。[结论]本方法可准确测定活血止痛片中三七皂苷R₁和人参皂苷Rg₁的含量。

Abstract:

[Objective] To perfect the quality standard of notoginsenoside R₁ and ginsenoside Rg₁ in the Huoxue Zhitong tablet. [Methods] The chromatographic separation was performed on a Kromasil C₁₈ column with a linear gradient elution of acetonitrile-0.05% phosphoric acid,0~10 min,20% acetonitrile; 10~40 min,20% acetonitrile -30% acetonitrile. Detection wavelength was set at 203 nm; flow rate was 1.0 mL/min and column temperature was set at 30 ℃.[Results] Notoginsenoside R₁ and ginsenoside Rg₁ had a good linearity in the range of 0.207 2~1.036 μg and 0.8296~4.148 μg,The average recovery of this method was 96.1%,97.3%,RSD=2.02%,2.34%(n=5)respectively.[Conclusions] The procedure is simple and reliable and the results were stable and reproducible. The established method can determine notoginsenoside R₁ and ginsenoside Rg₁ in the Huoxue Zhitong tablet accurately.