芪玄益心胶囊对冠脉结扎大鼠心肌肝细胞生长因子及血管内皮生长因子mRNA表达的影响

Effect of Qixuanyixin capsule on the expression of mRNA of HGF and VEGF in rats with coronary artery ligation

关键词:  芪玄益心胶囊  心肌梗死  动物模型  原为杂交  肝细胞生长因子  血管内皮生长因子  大鼠

Key words:  Qixuanyixin capsule  myocardial infarction  HGF  VEGF

摘要:

[目的]评价芪玄益心胶囊对冠脉结扎大鼠心肌梗死的治疗作用,并探讨其机制。[方法]建立大鼠冠脉结扎心肌梗死模型,将模型大鼠随机分为麝香保心丸组、芪玄益心胶囊高、中、低剂量组、模型组,并设空白对照组。观察各组模型心肌梗死率和心肌组织肝细胞生长因子(HGF)mRNA、血管内皮生长因子(VEGF)mRNA的表达,并与空白组对照。[结果]模型组及各治疗组与空白组比较心肌梗死率、HGFmRNA、VEGFmRNA表达均显著增高(P<0.05)。芪玄益心胶囊各剂量组与模型组比较心肌梗死率明显降低,VEGFmRNA、HGFmRNA表达明显增高(P<0.05),以高剂量组变化最显著,高剂量组在降低心肌梗死率,增高VEGFmRNA、HGFmRNA表达方面均优于麝香保心丸组(P<0.05)。[结论]芪玄益心胶囊能够降低心肌梗死率,并推测芪玄益心胶囊抗心肌梗死的作用与增加VEGFmRNA、HGFmRNA表达相关。

Abstract:

[Objective] To evaluate the therapeutic effect of Qixuanyixin capsule in rats with myocardial infarction and explore the effect mechanism. [Methods] The model of myocardial infarction was induced by ligating the coronary artery of rats. They were randomly divided into Shexiangbaoxin pill group, high, middle and low dose of capsule Qixuanyixin group, placebo group and control group. The incidence rate of myocardial infarction and the expression of mRNA of HGF and VEGF in myocardial tissue were observed. [Results] Compared to blank group, the rate of myocardial infarction and the expression of mRNA of HGF and VEGF in model and each treatment group were higher(P<0.05). The rate of myocardial infarction and the expression of mRNA of HGF and VEGF in myocardial tissue were lower than that in each dose of capsule Qixuanyixin(P<0.05), with the most obvious effect in high dose group, and it was superior than Shexiangbaoxin pill (P<0.05). [Conclusions] Qixuanyixin capsule can decrease the incidence rate of myocardial infarction at is speculated that the anti-infarction effect was related with the increased expression of mRNA of HGF and VEGF.