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| 靛玉红对肿瘤细胞抑制作用的研究及相关机制探讨 |
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吴琦玮1, 葛忠良2, 高月2, 张丽3
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1.北京市石景山医院消化内科, 北京, 100043;2.军事医学科学院放射与辐射医学研究所, 北京, 100850;3.北京中医药大学, 北京, 100029
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| 摘要: |
| [目的]探讨靛玉红对与细胞周期依赖性蛋白激酶(CDK)、Src激酶有关的人类肿瘤细胞生长的抑制作用。[方法]用细胞集落培养方法验证靛玉红对的人白血病细胞株K562、人胃癌细胞株、人淋巴瘤细胞株、人宫颈癌细胞株Heal、人早幼粒白血病细胞株HL-60、人胆囊癌、肝癌、肝门胆管癌细胞株的影响。用四甲基偶氮唑盐(MTT)方法观测靛玉红对K562、胆囊癌、肝门胆管癌细胞株的影响。[结果]靛玉红对这几种肿瘤存在广泛的抑制作用。[结论]靛玉红及其衍生物可能成为有CDK、Src激酶参与的肿瘤的新一代抗肿瘤药物。 |
| 关键词: 靛玉红 肿瘤 细胞周期 激酶 |
| DOI：10.11656/j.issn.1672-1519.2008.01.26 |
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| Inhibitory effect of indirubin on growth of some cancer cells and its mechanism |
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WU Qi-wei1, GE Zhong-liang2, GAO Yue2
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1.Beijing Shijingshan Hospital, Beijing 100043, China;2.Institute of Radiation Medicine of Academy of Military Medical Sciences, Beijing 100850, China;3.Beijing University of TCM, Beijing 100029, China
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| Abstract: |
| [Objective] The inhibitory effect of indirubin on cell growth was studied in some human tumor cell lines relating with cyclin-dependent kinases(CDK) or Src kinase. [Methods] The cell growth, such as leukemia cell line(K562), gastric cancer cell line, lymphoma cell line, uterine cervix cancer cell line, promyelocyte leukemia cell line(HL-60), carcinoma of gallbladder cell line, hepatocarcinoma cell line, hilar cholangiocarcinoma cell line was proved inhibited by indirubin using clone cell culture method. The influence of indirubin on the K562 cell line, the gallbladder cancer cell and hilar cholangiocarcinoma cell line was observed with MTT method. [Results] The inhibitory effect of indirubin was verified extensively in some cancer cell growth. [Conclusions] Indirubin and its derivatives may become the new anti-tumor medicine for therapy of cancers that have CDK, Src kinase. |
| Key words: indirubin cancer cell cycle kinase |
