益气养阴活血化痰汤对肺纤维化大鼠干预作用的实验研究

Experimental study on the intervention effect of Yiqiyangyin huoxuehuatan decoction in rat with pulmonary fibrosis

关键词:  肺纤维化  肺指数  转化生长因子-β  大鼠

Key words:  Pulmonary fibrosis  Lung index  Transforming growth factor beta  Sprague-Dawley rat

摘要:

[目的]探讨益气养阴活血化痰汤对肺纤维化大鼠的干预作用。[方法]将40只大鼠随机分为正常组(A)、模型组(B)、中药组(C)和西药组(D),每组各10只,并分组给与治疗。建立博莱霉素诱导的大鼠肺纤维化动物模型,观察大鼠体质量、肺指数等一般状况,检测肺组织转化生长因子-β₁(TGF-β₁)的表达情况。[结果]与正常组比较,模型组体质量降低、肺指数增加、TGF-β₁高表达,P<0.05;益气养阴活血化痰汤可有效地增加大鼠体质量、抑制肺指数的升高、降低TGF-β₁的过度分泌和表达,与模型组比较有显著性差异(P<0.05)。[结论]益气养阴活血化痰汤可改善博莱霉素诱导肺纤维化大鼠的一般状况,并通过抑制其肺组织TGF-β₁的表达,以达到抗肺纤维化的作用。

Abstract:

[Objective] To discuss the intervention effect of Yiqiyangyin(benefiting vital QI and nourishing YIN) Huoxuehuatan (activating blood circulation and resolving phlegm) decoction, a traditional Chinese medicine (TCM), on rats with pulmonary fibrosis (PF). [Methods] Forty rats were randomly divided into four groups: normal group(A), model group(B), TCM group (C) and Western medicine group(D). Bleomycin-induced PF was established in rats. The rat’s weight, lung index and general situation were observed and the expression of lung tissue transforming growth factor-β₁ (TGF-β₁) was detected. [Results] Compared with the normal group, the body weight was reduced, lung index was increased, expression of TGF-β₁ was high in rats of model group(P<0.05). Compared with the model group, TCM group was effective in increasing body weight of rats, inhibiting the increase of lung index. It could reduce the excessive secretion and expression of TGF-β₁ with significant differences (P<0.05). [Conclusions] Yiqiyangyin Huoxuehuatan decoction may improve the general situation of rats with bleomycin-induced PF, and achieves the role of against PF through inhibiting the expression of TGF-β₁ of lung tissues.