| 摘要: |
| [目的]研究丹酚酸B(SalB)对心肌梗死大鼠心肌细胞凋亡的抑制作用及分子机制。[方法]结扎冠脉左前降支复制大鼠心肌梗死模型,假手术组作对照,连续灌胃给药7d。采用流式细胞术磷脂结合蛋白5(AnnexinⅤ)-异硫氰酸荧光素/碘化丙啶(FITC/PI)法检测心肌细胞凋亡情况,并用实时荧光定量反转录—多聚酶链式反应(RT-PCR)技术检测凋亡相关调控通路的基因表达。[结果]丹酚酸B(SalB)可以显著降低心肌细胞凋亡率。SalB可以显著降低Bax、信号转导和转录活化因子(STAT-3)的表达,有降低白细胞介素-6(IL-6)表达的趋势。[结论]SalB可以有效抑制心肌细胞凋亡,改善心肌梗死后的心脏状况。 |
| 关键词: 丹酚酸B 心肌梗死 心肌细胞 凋亡 |
| DOI：10.11656/j.issn.1672-1519.2008.04.21 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划“973”课题资助项目(2005CB523404);天津市科委自然科学基金项目(07JCYB-JC16900) |
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| Effect of salvianolic acid B on apoptosis of myocardial cells in rats with nyocardial infarction |
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ZHANG Meng1, WANG Yi2, WANG Hong2
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1.The TCM College of Tianjin University of TCM, Tianjin 300193, China;2.The Academy of TCM of Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the inhibitory effects and mechanism of Salvianolic acid B (Sal B) on apoptosis of myocardial cells in rats with acute myocardial infarction. [Methods] We produced myocardial infarction in anaesthetized rats by occluding the left descending coronary artery. Sham-operated rats served as controls. We assayed the myocardial cell apoptosis and the gene expressions by flow cytometry and real-time RT-PCR. [Results] Sal B can decrease the number of apoptotic myocytes and decrease the expression of IL-6, Bax and STAT-3. [Conclusion] Sal B can limit the myocardial cell apoptosis after infarction and the mechanism may be related to the down regulation of the apoptosis relative genes expression. |
| Key words: salvianolic acid B myocardial infarction myocardial cell apoptosis |
