中药对于实验大鼠急性心肌梗死的干预作用

The intervention effect of TCM on acute myocardial infarction in rats

关键词:  急性心肌梗死  心复康  丹酚酸B

Key words:  acute myocardial infarction  XFK  SalB

摘要:

[目的]通过制作大鼠急性心肌梗死模型,探讨中药对大鼠心肌梗死的干预作用。[方法]制作雄性Wistar大鼠实验性心肌梗死模型;利用中药复方心复康及中药单体丹酚酸B对大鼠进行干预;2周后采用硝基四氮唑蓝(N-BT)染色分别评价大鼠心肌梗死面,测量室壁厚度;超声心动仪检测大鼠心功能;免疫组化法检测大鼠心肌细胞增殖情况及心肌再生血管情况。[结果]N-BT染色及超声心动图结果:各治疗组心肌梗死面积均有所下降,梗死区厚度有所增加,心功能有所改善(P<0.05);增殖细胞核杭原(PCNA)免疫组化结果与模型组比较,治疗组PCNA阳性细胞数均有增加(P<0.05);CD34免疫组化结果与模型组比较,治疗组新生毛细血管数均有增加(P<0.05)。[结论]在心肌梗死大鼠模型中,应用中药复方心复康及单体丹酚酸B治疗可以减少梗死区面积,增加梗死区心室厚度,促进梗死周边区域血管再生,改善心功能。

Abstract:

[Objective] To explore the mechanism of the Chinese herbal medicine having improving effect on the heart function in the model of rats with acute myocardial infarction(AMI). [Methods] The AMI was induced in rats by ligating the middle segment of left anterior descending coronary artery. The Chinese herbal medicine Xinfukang(XFK) and SalB were used to intervene the disease course of the rats. The rat’s heart function was examined, and the infarct size was evaluated with N-BT and the expression of PCNA and CD34 was examined by immunohistochemical method at the end of two weeks. [Results] The infarct area of each group was declined and the infarct thickness of each group was increased, the heart function was improved.(P<0.05). The consequence of PCNA, the amount of positive cells in the capillary vessels of each group were increased (P<0.05). The consequence of CD34, the amount of positive cells of each group were increased ( P<0.05). [Conclusion] In AMI rats, the Chinese herbal medicine can reduce the infarct area, increase the thickness of infarct ventricle, accelerate regeneration of blood vessel, promote the multiplication of cardiac muscle cells and improve the heart function.