| 摘要: |
| [目的]通过对观察丹酚酸A对缺氧/复氧(H/R)损伤条件下脑微血管内皮细胞细胞间黏附因子-1(CAM-1)表达的影响,探讨丹酚酸A对缺血再灌注急性期脑保护作用机制。[方法]培养BMEC,丹酚酸A预处理20h后制作(H/R)/模型,逆转录-聚合酶链式反应(RT-PCR)法检测BMEC细胞间黏附因子-1ICAM-1mRNA的表达。以尼莫地平作为阳性对照药。[结果]模型组与空白对照组相比ICAM-1mRNA表达明显升高(P<0.01)。丹酚酸A组ICAM-1mRNA表达低于模型组(P<0.05)。[结论]丹酚酸A可降低H/R损伤条件下BMECICAM-1mRNA表达的影响,提示阻抑粒细胞黏附作用是其脑保护作用机制之一。 |
| 关键词: 丹酚酸A 缺氧/复氧(H/R) 细胞间黏附因子-1(ICAM-1) 逆转录-聚合酶链式反应(RT-PCR) |
| DOI：10.11656/j.issn.1672-1519.2008.05.26 |
| 分类号: |
| 基金项目:国家自然基金资助项目(30572444) |
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| Effect of salvianolic acid A on ICAM-1 expression after Hypoxia/ Reoxygenation injury in brain microvascular endothelial cells |
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JIANG Min, GUO Li-ping, ZHONG Wang-yi
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Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the action of Sal A on the expression of cellular adhesion molecules after hypoxia/reoxygenation (H/R) injury in brain microvascular endothelial cells (BMEC). [Methods] After pretreating with Sal A for 20h, the H/R was successfully established H/R on BMEC. Then the expression of ICAM-mRNA was evaluated by RT-PCR method. Nimodipine served as positive control.[Results] Compared with control, the expression of ICAM-mRNA of H/R model enhanced significantly (P<0.01). Sal A could downregulate the expression of ICAM-1 in the H/R model on BMEC (P<0.05). [Conclusion] Sal A can downregulate the expression of ICAM-1 in the H/R injured BMEC, suggesting that the molecular mechanism of Sal A in brain protection was based on down-regulating the expression of cellular adhesion molecules. |
| Key words: Salvianolic Acid A hypoxia/reoxygenation ICAM-1 RT-PCR |
