心身1号方对慢性应激模型大鼠血清SOD、MDA及结肠黏膜病理形态学的影响

Effect of Xinshen No.1 on the expression of serum SOD, MDA and pathology of colon mucous membrane in rats with chronic stress

关键词:  心身1号方  慢性应激  超氧化物歧化酶  丙二醛

Key words:  Xinshen No.1  chronic stress rats  SOD  MDA

摘要:

[目的]观察中药心身1号方对慢性应激模型大鼠血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及对大鼠结肠黏膜病理形态学的影响。[方法]建立SD大鼠的慢性应激模型,实验动物随机分为:对照组、模型组、心身1号高、中、低剂量组,每组8只。采用酶标仪检测大鼠血清中SOD活性和MDA含量,同时光镜下观察各组大鼠结肠黏膜病理形态学改变。[结果]模型组大鼠血清SOD活性低于正常对照组,两者差异有统计学意义(P<0.05),心身1号高、中、低剂量各组大鼠血清SOD活性均高于模型组,差异有统计学意义(P<0.05)。与对照组相比较,模型组、心身1号高、中、低剂量组大鼠血清MDA含量均升高,差异有统计学意义(P<0.05),与模型组相比,心身1号高、中、低剂量组大鼠血清MDA含量均降低,差异有统计学意义(P<0.05)。[结论]中药心身1号方可以降低大鼠血清MDA含量,升高SOD活性,提高大鼠抗氧化能力,减轻脂质过氧化损伤,同时对应激状态下大鼠结肠黏膜病理形态也有较好的改善作用。

Abstract:

[Objective] To observe the effect of Xinshen No.1 (treating mental disease) on the expression of serum SOD, MDA and the pathologic changes of colon mucous membrane in rats with chronic stresss.[Methods] Chronic stress was established in Wistar rats.They wererandomly divided into five groups:blank group, model group, Xinshen No.1-treated stress groups (high, middle and low dosage) with 8 rats ineach group.Serum SOD, MDA were detected with enzyme-scale meter and the pathologic changes of colon mucous membrane were observed with light microscope.[Results] The serum SOD activity of the model group was lower than that of the blank group (P<0.05).The SODactivity of Xinshen No.1-treated stress groups (high, middle and low dosage) was higher than that of model group (P<0.05).Compared withblank group, the serum MDA of model group, Xinshen No.1-treated stress groups (high, middle and low dosage) was higher (P<0.05).Compared with model group, it was decreased (P<0.05) in Xinshen No.1-treated stress groups (high, middle and low dosage).[Conclusion] Thetraditional Chinese medicine Xinshen No.1 can decrease the MDA contents in serum, heighten the SOD activity and increase the rats antioxygen capability and lessen lipid peroxidation damage and can also improve the pathologic morphology of colon mucous membrane.