腹泻型肠易激综合征大鼠模型的建立

Inducing of irritable bowel syndrome with diarrhea in rats

关键词:  腹泻型肠易激综合征  番泻叶  灌胃  肢体束缚

Key words:  IBS-D  Folium sennae  oral administration  limbs tied

摘要:

[目的]在传统束缚应激+番泻叶灌胃方法的基础上进行改良,旨在建立简便易行的腹泻型肠易激综合征大鼠模型。[方法]将大鼠随机分组,空白对照组(N)未做任何处理。单纯番泻叶组(F)灌服最佳浓度番泻叶水煎剂,不行束缚。单纯束缚组(S)灌服高压消毒饮用水,番泻叶联合束缚应激组(F+S)灌服最佳浓度番泻叶水煎剂,以上两组均行肢体束缚。观察各实验组体质量变化、排便情况等。[结果]N和S无稀便排出,而F、F+S两组均能排出不成形的稀软便,以F+S组明显(P<0.05)。与N组相比较,其余3组大鼠平均体质量增长率均有显著性差异(P<0.01),尤以F+S呈明显负增长趋势(P<0.01)。S与F+S可见免疫器官明显萎缩,脏体指数降低,与N、F两组相比较差异具有显著性(P<0.05)。[结论]肢体束缚应激联合低浓度番泻叶灌胃方法,可以成功复制肠易激综合征(IBS-D)大鼠模型。

Abstract:

[Objective] To establish a stable and easy model of irritable bowel syndrome with diarrhea(IBS-D) in rats on the basis of improved traditional method. [Methods] The rats were randomly divided into some groups:the normal(N,without any intervention),the Folium Cassiae only(F,oral infusion with Folium Cassiae without restriction),the restriction only(S),the Folium Cassiae and restriction(F+S,oral administration folium cassiae plus restriction). We observed and recorded the general status,the body weight and passage everyday. [Results] All the groups were in a good status. Group F and F+S were higher in the percentage of the loose stools than that of other groups,and F+S was better(P<0.05). The other groups were lower in the percentage of the increased body weight than that of group N(P<0.01),and the growth tendency of group F+S was appeared as negative increasing obviously(P<0.01). [Conclusion] It is proved that the improved method can reproduce IBS-D in rats successfully.