蕨麻正丁醇部位对缺氧损伤心肌细胞的保护作用

Protective effect of the silverweed cinquefoil root n-butanol fraction on the cardiomyocyte injured by hypoxia

关键词:  蕨麻  正丁醇部位  缺氧  抗氧化

Key words:  silverweed cinquefoil root  n-butanol fraction  hypoxia  anti-oxide

摘要:

[目的]研究蕨麻正丁醇部位对缺氧损伤心肌细胞的保护作用。[方法]建立大鼠原代培养心肌细胞缺氧损伤模型,采用噻唑蓝(MTT)法检测心肌细胞代谢活力;胎盼兰染色法检测细胞成活率;比色法测定细胞外乳酸脱氢酶(LDH),肌酸激酶(CK)活性及细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。[结果]与模型组比较,蕨麻正丁醇提取部位各剂量组细胞代谢活力及细胞成活率显著提高(P<0.01),并均可显著减少缺氧损伤引起的LDH、CK的外漏量(P<0.01),提高细胞SOD活性(P<0.01),减少MDA的产生(P<0.01或P<0.05)。[结论]蕨麻正丁醇部位具有抗心肌缺氧损伤作用,其途径之一可能与其抗自由基氧化的能力有关。

Abstract:

[Objective] To study the protective effect of the silverweed cinquefoil root n-butanol fraction on the cardiomyocyte injured by hypoxia. [Methods] Myocardiocyte injured by hypoxia was established in rats in vitro. The metabolic activity and the survive rate of the myocardiocytes were determined respectively with MTT and Trypan blue stain. The activities of lactate dehydrogenase(LDH) and creatine kinase(CK) out of cells and superoxide dismutase(SOD) in cells,and the level of malondialdehyde(MDA) in cells were measured by spectrophotometry. [Results] Comparing with model group,the metabolic activity and the survival rate of cardiomyocytes were increased significantly in all groups treated by silverweed cinquefoil root n-butanol fraction(P<0.01),the releases of LDH,CK from myocardiocytes resulted from hypoxia were reduced(P<0.01),the activity of SOD was improved(P<0.01) and the production of MDA was inhibited in cells during hypoxia injury. [Conclusion] The silverweed cinquefoil root,n-butanol fraction exerts a remarkable protective effect on cardiomyocyte during hypoxia by improving the ability of clearing oxygen free radial in cardiac myocyte and inhibiting the oxide injury to membrane. It suggests that the silverweed cinquefoil root,n-butanol fraction can inhibit injury of cardiac muscles during hypoxia and this may be related with its anti-oxide ability.