益气活血法对单侧输尿管梗阻大鼠肾小管上皮细胞转分化的影响

Effect of Qi supplement and Blood activation therapy on treating TEMT in UUO rats

关键词:  益气活血法  肾小管上皮细胞转分化  转化生长因子-β1  α-平滑肌肌动蛋白

Key words:  Qi supplement and Blood activation therapy  tubular epithelial myofibroblast transdifferentiation  TGF-β1  Smad2/3  α-SMA

摘要:

[目的]观察益气活血法中药方剂对单侧输尿管梗阻大鼠肾组织转化生长因子-β1(TGF-β1)、果蝇MAD类似基因2/3(Smad2/3)和α-平滑肌肌动蛋白(α-SMA)表达的调节,探讨其对肾小管上皮细胞转分化的影响。[方法]采用单侧输尿管结扎致肾间质纤维化大鼠模型。将30只大鼠随机分为6组:假手术组、模型组、中药大剂量组、中药中剂量组、中药小剂量组、西药组。术后14d,观察梗阻肾组织病理改变,用免疫组化方法检测肾组织TGF-β1、Smad2/3和α-SMA的表达。[结果]益气活血法能减轻肾间质纤维化程度,并能下调TGF-β1、Smad2/3和α-SMA的表达。[结论]益气活血法可通过下调TGF-β1、Smad2/3和α-SMA的表达,从而抑制大鼠肾小管上皮细胞转分化。

Abstract:

[Objective] To investigate the regulating effect of qi supplement and blood activation therapy method in TCM on expression of the TGF-β1, Smad2/3 and α-SMA in rats with unilateral ureteral obstruction and to explore the therapeutic action on tubular epithelial myofibroblast transdifferentiation. [Methods] The unilateral ureteral ligation was used to induce renal interstitial fibrosis in rats. They were divided into treatment groups and sham operation group with 5 rats in each. Thirty male rats were selected and randomly divided into the model group, the high, middle and low dose TCM groups, Western medicine group. At 14th postoperative day, the nephridial tissues of operating side were harvested to detect the change of pathology. The expressions of TGF-β1, Smad2/3 and α-SMA in renal tissue were examined by using immunohistochemistry method. [Results] The Qi supplement and blood activation therapy could lessen the renal interstitial fibrosis and down-regulate the expression of TGF-β1, Smad2/3 and α-SMA. [Conclusion] The Qi supplement and blood activation therapy can inhibit the tubular epithelial myofibroblast transdifferentiation by down-regulating the expression of TGF-β1, Smad2/3 and αSMA.