葛花醒酒汤抑制小鼠乙醇性肝纤维化的实验研究

Inhibiting effects of Traditional Chinese Medicine Gehua XingJiu decoction on alcohol hepatic fibrosis in mice

关键词:  葛花醒酒汤  肝纤维化  肝星形细胞  血清Ⅲ型前胶原肽  层粘连蛋白

Key words:  Gehua XingJiu Decoction  alcohol hepatic fibrosis  hepatic stellate cell  PⅢP  LN

摘要:

[目的]研究葛花醒酒汤对小鼠肝纤维化模型星形细胞活化、转型和血清Ⅲ型前胶原肽(PⅢP)、层粘连蛋白(LN)水平的影响,探讨葛花醒酒汤对小鼠乙醇性肝纤维化的防治作用。[方法]将60只小鼠随机分成对照组10只、模型组25只,治疗组25只,采用白酒灌胃[60°白酒,0.015mL/20(g·d)]的方法建立小鼠肝纤维化模型,12周末处死小鼠,观察肝组织病理形态改变;免疫组化法检测肝组织胶质纤维酸性蛋白(GFAP)、Desmin的表达;酶联免疫吸附(ELISA)方法检测血清PⅢP、LN水平变化。[结果]与对照组比较,模型组肝组织GFAP、Desmin表达增加(P<0.01),血清PⅢP、LN水平升高(P<0.05),纤维组织增生较明显。和模型组比较,治疗组肝组织GFAP、Desmin表达减少(P<0.01),血清PⅢP、LN水平降低(P<0.05),纤维组织增生不明显。[结论]葛花醒酒汤可以有效抑制小鼠乙醇性肝纤维化的发生和发展。

Abstract:

[Objective] To investigate the inhibiting effects of traditional Chinese medicine Gehua XingJiu Decoction on alcohol hepatic fibrosis in mice. [Methods] Sixty mice were randomly divided into three groups: model group, Gehua XingJiu Decoction prescription group, normal group. Alcohol hepatic fibrosis was induced in mice by stomach infusing with alcohol (60° white wine, 0.3ml/20g/d). After 12 weeks intervention, the variety of hepatic fiborsis index(GFAP, Desmin, PⅢP, LN) and liver tissue pathology were observed. [Results] After 12 weeks, compared with the normal mice, the model mice’ GFAP and Desmin increased(P<0.01), PⅢP, serum laminin in mice increased (P<0.05), and fibrosis were observed in pathology. Compared with the model’s mice, GFAP and Desmin in Gehua XingJiu decoction prescription group decreased (P<0.01), serum PⅢP, laminin decreased (P<0.05), and fibrosis was not obvious in pathology examination. [Conclusion] Gehua XingJiu Decoction has an active effect on inhibiting of the alcohol hepatic fibrosis in mice.