益气养阴方抗血管生成抑制肿瘤转移作用的研究

Study on inhibitory effect of cancer metastasis of reinforcing Qi and nourishing Yin prescription by antiangiogenesis

关键词:  益气养阴方  血管生成  自发转移

Key words:  reinforcing Qi and nourishing Yin prescription  angiogenesis  spontaneous metastasis

摘要:

[目的]研究益气养阴方抗血管生成抑制肿瘤转移的效应。[方法]制备接种肿瘤细胞的鸡胚尿囊膜(CAM)模型,采用血清药理学方法制备含药血清。观察含药血清对肿瘤细胞诱导CAM血管生成的影响,并与生理盐水血清组对照。另用小鼠经肌肉接种U14子宫颈癌细胞悬液,观察小鼠实体瘤的瘤质量、抑瘤率、肺部转移率、肺部转移瘤生长情况。[结果]益气养阴方含药血清可抑制肿瘤细胞诱导CAM血管生成,与生理盐水组比较差异具显著性。益气养阴方组的肺部转移结节较模型组明显减少,并且明显改善化疗对机体的免疫损伤。[结论]益气养阴方对肿瘤细胞诱导的CAM血管生成有明显抑制作用,并且益气养阴方对自发性转移模型有一定的抑制作用。

Abstract:

[Objective] To study the effect of reinforcing Qi and nourishing Yin prescription (RQNYP) on angiogenesis for inhibiting the metastasis. [Methods] Chicken CAM into that the tumor cells can be transplanted was adopted. The drug-contained serum was prepared using serum pharmacological method. Chicken embryos were hatched and then angiogenesis in tumor cells transplanted in CAM was observed after application of nutritionaldoses serum and saline serum. The situation of lung metastasis and hemorheology was observed in groups after intravenous injection in KM mice. [Results] The drugs-containing serum in rats could inhibit blood vessel formation in chicken chorioallantoi membrane transplanted with tumor cells. It was statistical significant when compared with that of saline. Compared with the control animals,the metastasis nodules of lung were decreased in RQNYP group animals. RQNYP group could also change the results of the hemorheologic determination. [Conclusion] RQNYP has an inhibitory effects on angiogenesis induced by tumor cells on CAM. It has an inhibitory effect on the metastasis of U14 tumor as well.