丹酚酸B对ApoE小鼠糖尿病动脉粥样硬化治疗作用

Therapy of salvianolic acid B on diabetic atherosderosis(AS) in almlilmprotein E-gene knockout mice

关键词:  丹酚酸B  载脂蛋白E基因敲除小鼠  糖尿病动脉粥样硬化

Key words:  salvianolic acid B  ApoE  diabetic atherosclerosis

摘要:

[目的]观察丹酚酸B对ApoE小鼠糖尿病动脉粥样硬化的治疗作用。[方法]将8周龄ApoE小鼠腹腔注射链脲佐菌素200mg/kg并高脂喂养12周,糖尿病动脉粥样硬化模型成功后,随机分为模型组、丹酚酸B组与洛伐他汀组。丹酚酸B组灌服丹酚酸B160mg/kg,洛伐他汀组灌以洛伐他汀2.3mg/kg,模型组灌服等量生理盐水。连续灌胃8周后,处死,取主动脉做形态学观察及图像分析。[结果]模型组动脉粥样硬化病变显著,多为粥样硬化期病灶,可见由大量泡沫细胞形成的脂纹脂斑期病变,也可见由大量泡沫细胞及胆固醇结晶形成的粥样斑块期病灶。丹酚酸B组与洛伐他汀组动脉粥样硬化病变较模型组明显减轻,多为早期粥样硬化,斑块面积显著小于模型组(P<0.05)。[结论]提示丹酚酸B有减轻ApoE小鼠糖尿病动脉粥样硬化作用。

Abstract:

[Objective] To observe the effect of Salvianolic acid B on diabetic atherosclerosis in Apolipoprotein E (ApoE)-gene knocked mice. [Methods] eight-week old mice were given intraperitoneal injection with stz and fed with high fat diet for 12-weeks to establish a diebatic atherosderosis model,then assigned them randomly into three groups:model group (fed with saline) Salvianolic acid B group (fed with Salvianolic acid B )and atorvastatin calcium positive control group (2.3 mg/kg everyday). After treating for 8 weeks,all the mice were killed and the aortas were stripped for morphological research and photograph analysis. [Results] In model group,some plaques were bigger,merged into flake,aortic wall thickened diffusively. Compared with that,In Salvianolic acid B treatment group and Lovastatin positive control group,the aortic sinus had a more limited plaque lesion. Morphological research showed that As lesions were found in aortas of model group and Salvianolic acid B group. The lesions of model group were more serious than those of Salvianolic acid B group. [Conclusion] Salvianolic acid B can relieve the diabetic As lesions of the ApoE gene knockout mice.