| 摘要: |
| [目的]建立双黄连冻干粉高效液相色谱法(HPLC)指纹图谱检测方法,对其特征色谱峰进行归属研究。[方法]将双黄连冻干粉HPLC指纹图谱与各组成单味药代表成分的HPLC指纹图谱进行比较分析,利用各色谱峰的相对保留时间,对双黄连冻干粉主要色谱峰进行归属和定性。[结论]HPLC指纹图谱中分离得到21个色谱峰,确定了指纹图谱中7个特征色谱峰的归属,分别为:绿原酸、连翘酯苷、黄芩苷、连翘苷、木犀草素、汉黄芩苷和黄芩素。[结论]利用指纹图谱技术对双黄连冻干粉中主要特征峰进行定性研究,可为实现对药品的质量控制进行整体描述和评价奠定基础。 |
| 关键词: 双黄连冻干粉 高效液相指纹图谱 特征色谱峰 |
| DOI：10.11656/j.issn.1672-1519.2009.06.23 |
| 分类号: |
| 基金项目:国家科技部中医药行业专项(200707011) |
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| Classified research of the characteristic chromatographic peaks of Shuanghuanglian Powder-Injection in its fingerprints and HPLC |
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GUO Jie1, SONG Dian-rong1, WANG Yue-fei2
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1.Gynecology Department of the Second Hospital Affiliated to Tianjin University of TCM, Tianjin 300150, China;2.The TCM Research Centre of Tianjin University of TCM, Key Laboratory of Pharmacology of Traditional Chinese Medincal Formuloe, Ministry of Education,Tianjin 300193, China
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| Abstract: |
| [Objective] To establish the fingerprint of Shuanghuanglian Powder-Injection by high performance liquid chromatography (HPLC), and to qualitatively analyze the marker peaks of Shanghuanglian Powder-Injection. [Methods] The HPLC fingerprint of Shuanghuanglian Powder-Injection was compared with those reference substances of corresponding component herbs. The relative retention time of each peak, and some reference substances were used in the analyses. [Results] Twenty-one marker peaks were separated by HPLC, seven marker peaks were confirmed to the Shuanghuanglian powder-injection respectively, as follow: caffeotannic acid, forsythoside, baicalin, forsythin, luteolin, wogonoside and baicalein. [Conclusion] Some marker peaks and their synergy origins can be identified by chromatographic fingerprint technology,which can provide much useful information to the quality control of Shuanghuanglian Powder Injection. |
| Key words: Shuanghuanglian Powder-Injection HPLC fingerprint marker peaks |
