补肾调肝法对治疗自身免疫性卵巢早衰小鼠的实验研究

Experimental research on invigorating kidney and regulating liver for autoimmune premature ovarian failure in mice

关键词:  补肾调肝法  自身免疫性  卵巢早衰

Key words:  invigorating kidney and regulating liver  autoimmune  premature ovarian failure

摘要:

[目的]旨在探讨中医药在防治卵巢早衰时的思路和方法,以期给临床治疗提供强有力的依据。[方法]参照文献研究随机将60只小鼠分为6组制作免疫性小鼠卵巢早衰模型:造模成功后第6天开始各药物组灌胃给药。连续灌胃4周后摘眼球取血,处死小鼠,取组织待测。采用放免法测定小鼠血清促卵泡生成素(FSH)、促黄体生成素(LH)及雌二醇(E₂)的含量。[结果]1)西药组灌胃4周后FSH、LH值明显降低,E₂值明显升高,与中药各组有显著性差异(P<0.05)。2)中药大剂量组FSH、LH值降低幅度虽不及西药组,但FSH、LH与模型组比较结果有显著性差异,结果说明灌胃4周后中药大剂量组能恢复部分卵巢功能,且中药长期服用不良反应较西药明显减少,仍可说明其意义及可行性。[结论]补肾调肝中药方剂对自身免疫性卵巢早衰小鼠有一定的治疗作用,其治疗机制主要通过纠正紊乱的生殖内分泌轴,恢复部分的卵巢功能,改善临床症状,进一步对免疫性卵巢早衰起到一定的防治作用。

Abstract:

[Objective] To explore the way of preventing premature ovarian failure with traditional Chinese medicine and provide the basis for clinical practice.[Methods] Based on the reference literatures,60 mice were randomly divided into 6 groups for the models of premature ovarian failure.The animals in each group were fed with different medicine separately on the 6th day.After continuously feeding for four weeks,the rats were killed and their blood and tissue were observed.The serum content of FSH,LH and E2 was measured.[Results] Significantly decreased FSH and LH and increased E2 were found in the group with Western medicine compared to the group with Chinese medicine (P<0.05).Although the decrease of FSH and LH was lower in the group with high dose of Chinese medicine compared to the group with Western medicine there was a significant difference in that group compared to the control group,indicating that high dose Chinese medicine is helpful for the ovary function with fewer side effects.[Conclusion] The Chinese prescription of invigorating the kidney and regulating the liver can be effective for the rats with autoimmune premature ovarian failure.It is helpful for preventing autoimmune premature ovarian failure through regulating the disorder of reproduction-endocrine-axis,regaining the function of ovary and improving clinical syndromes.