| 摘要: |
| [目的]考察胆红素的稳定性,为桂蒲肾清片中胆红素的含量测定方法提供依据。[方法]采用高效液相色谱(HPLC)法测定胆红素的含量,并进行了方法学考察;通过紫外光谱法和HPLC法测定胆红素日光照射前后的变化情况。[结果]胆红素不稳定,在避光的条件下,0.5h峰面积即下降1.6%,但方法学考查表明:精密度(RSD=1.2%)、重复性(RSD=2.0%)及回收率(96.0%,RSD=0.8%)可满足实验要求;胆红素对光非常敏感,日光下3h后紫外光谱完全变化,通过液相色谱未能检出胆红素。[结论]HPLC法可以作为胆红素的测定方法,但应注意避光操作和快速测定。 |
| 关键词: HPLC 胆红素 稳定性 |
| DOI：10.11656/j.issn.1672-1519.2010.01.32 |
| 分类号: |
| 基金项目: |
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| Effect of light on stability of cholerythrin and content of cholerythrin in the Guipu Shenqing tablet |
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ZHANG Dong-fang1, ZHOU Mei-huan2, YUAN Chang-ji1
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1.Pharmaceutical Science College, Chinese Medical University, Shenyang 110001, China;2.Shenyang North-east DaYaoFang Corporate Chain, Shenyang 110023, China
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| Abstract: |
| [Objective] To investigate the stability of cholerythrin and measure its content in the Guipu Shenqing tablet.[Methods] Cholerythrin was determined by HPLC.The amount of cholerythrin before and after the explosion of sunlight was compared by HPLC and UV spectrum.[Results] Cholerythrin was not stable and its peak area decreases for 1.6% in half an hour without light.It showed that the precision (RSD=1.2%),reproducibility (RSD=2.0%) and recovery rate could reach the need of experiments.Cholerythrin was sensitive to sunlight.It completely vanishes in sunlight for 3 hours and no cholerythrin was found by HPLC.[Conclusion] HPLC could be used to measure the content of cholerythrin and the drug should be prepared quickly with no light. |
| Key words: HPLC cholerythrin stability |
